Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 155 (2009), 1004-1015; DOI  10.1099/mic.0.026781-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Keith, K. E.
Right arrow Articles by Valvano, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Keith, K. E.
Right arrow Articles by Valvano, M. A.
Agricola
Right arrow Articles by Keith, K. E.
Right arrow Articles by Valvano, M. A.
Microbiology 155 (2009), 1004-1015; DOI  10.1099/mic.0.026781-0
© 2009 Society for General Microbiology

Delayed association of the NADPH oxidase complex with macrophage vacuoles containing the opportunistic pathogen Burkholderia cenocepacia

Karen E. Keith1, Daniel W. Hynes1, Judith E. Sholdice1 and Miguel A. Valvano1,2

1 Infectious Diseases Research Group, Siebens-Drake Research Institute, Department of Microbiology and Immunology, University of Western Ontario, London, ON N6A 5C1, Canada
2 Department of Medicine, University of Western Ontario, London, ON N6A 5C1, Canada

Correspondence
Miguel A. Valvano
mvalvano{at}uwo.ca

Burkholderia cenocepacia causes chronic lung infections in patients suffering from cystic fibrosis and chronic granulomatous disease. We have previously shown that B. cenocepacia survives intracellularly in macrophages within a membrane vacuole (BcCV) that delays acidification. Here, we report that after macrophage infection with live B. cenocepacia there is a ~6 h delay in the association of NADPH oxidase with BcCVs, while heat-inactivated bacteria are normally trafficked into NADPH oxidase-positive vacuoles. BcCVs in macrophages treated with a functional inhibitor of the cystic fibrosis transmembrane conductance regulator exhibited a further delay in the assembly of the NADPH oxidase complex at the BcCV membrane, but the inhibitor did not affect NADPH oxidase complex assembly onto vacuoles containing heat-inactivated B. cenocepacia or live Escherichia coli. Macrophages produced less superoxide following B. cenocepacia infection as compared to heat-inactivated B. cenocepacia and E. coli controls. Reduced superoxide production was associated with delayed deposition of cerium perhydroxide precipitates around BcCVs of macrophages infected with live B. cenocepacia, as visualized by transmission electron microscopy. Together, our results demonstrate that intracellular B. cenocepacia resides in macrophage vacuoles displaying an altered recruitment of the NADPH oxidase complex at the phagosomal membrane. This phenomenon may contribute to preventing the efficient clearance of this opportunistic pathogen from the infected airways of susceptible patients.

Abbreviations: AT, 3-amino-1,2,4-triazole; Bcc, Burkholderia cepacia complex; BcCV, Burkholderia cenocepacia-containing vacuole; CF, cystic fibrosis; CFTR, cystic fibrosis transmembrane conductance regulator; CGD, chronic granulomatous disease; DMEM, Dulbecco's modified Eagle medium; DPI, diphenyleneiodonium chloride; FBS, fetal bovine serum; mRFP1, monomeric red-fluorescent protein 1; NBT, nitro blue tetrazolium; PMA, phorbol 12-myristate 13-acetate; ROS, reactive oxygen species




This article has been cited by other articles:


Home page
 MicrobiologyHome page
M. S. Saldias and M. A. Valvano
Interactions of Burkholderia cenocepacia and other Burkholderia cepacia complex bacteria with epithelial and phagocytic cells
Microbiology, September 1, 2009; 155(9): 2809 - 2817.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Ortega, A. Silipo, M. S. Saldias, C. C. Bates, A. Molinaro, and M. A. Valvano
Biosynthesis and Structure of the Burkholderia cenocepacia K56-2 Lipopolysaccharide Core Oligosaccharide: TRUNCATION OF THE CORE OLIGOSACCHARIDE LEADS TO INCREASED BINDING AND SENSITIVITY TO POLYMYXIN B
J. Biol. Chem., August 7, 2009; 284(32): 21738 - 21751.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.