Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 155 (2009), 1164-1169; DOI  10.1099/mic.0.025114-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, Z.
Right arrow Articles by Walker, V. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, Z.
Right arrow Articles by Walker, V. K.
Agricola
Right arrow Articles by Wu, Z.
Right arrow Articles by Walker, V. K.
Microbiology 155 (2009), 1164-1169; DOI  10.1099/mic.0.025114-0
© 2009 Society for General Microbiology

Characterization and recombinant expression of a divergent ice nucleation protein from ‘Pseudomonas borealis’

Zhongqin Wu1, Lei Qin1 and Virginia K. Walker1,2

1 Department of Biology, Kingston, ON K7L 3N6, Canada
2 Department of Microbiology and Immunology, Kingston, ON K7L 3N6, Canada

Correspondence
Virginia K. Walker
walkervk{at}queensu.ca

Isolates of ‘Pseudomonas borealis’ were recovered after ice-affinity selection of summer-collected soils. ‘P. borealis’ DL7 was further characterized and shown to have ice nucleation activity (INA), a property that allows the crystallization of ice at temperatures close to the melting point, effectively preventing the supercooling of water. INA was optimally detected after culturing at temperatures consistent with psychrophilic growth. The sequence encoding the ‘P. borealis’ ice nucleation protein (INP) was obtained using both PCR and chromosome walking. When expressed in Escherichia coli, the resulting inaPb recombinants had INA. The ‘P. borealis’ sequence, dubbed inaPb, is clearly related to previously cloned INP genes, but it shows greater divergence. Sequence analysis suggests that there are two opposite flat surfaces, one relatively hydrophobic that likely serves as an ice template, and the other that could function as a complementary face to facilitate interprotein interaction for ice-step formation.

Abbreviations: INA, ice nucleation activity; INP, ice nucleation protein

The GenBank/EMBL/DDBJ accession number for the sequence of the ‘P. borealis’ INP gene and protein is EU573998.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.