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Microbiology 155 (2009), 1516-1522; DOI  10.1099/mic.0.026823-0
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Microbiology 155 (2009), 1516-1522; DOI  10.1099/mic.0.026823-0
© 2009 Society for General Microbiology

Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis

Olivia L. Champion1, Ian A. M. Cooper2, Sarah L. James1, Donna Ford2, Andrey Karlyshev3,{dagger}, Brendan W. Wren3, Melanie Duffield2, Petra C. F. Oyston2 and Richard W. Titball1

1 School of Biosciences, University of Exeter, Geoffrey Pope Building, Stocker Road, Exeter EX4 4QD, UK
2 Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK
3 London School of Hygiene and Tropical Medicine, Department of Infectious and Tropical Diseases, Keppel Street, London WC1E 7HT, UK

We report that larvae of the wax moth (Galleria mellonella) are susceptible to infection with the human enteropathogen Yersinia pseudotuberculosis at 37 °C. Confocal microscopy demonstrated that in the initial stages of infection the bacteria were taken up into haemocytes. To evaluate the utility of this model for screening Y. pseudotuberculosis mutants we constructed and tested a superoxide dismutase C (sodC) mutant. This mutant showed increased susceptibility to superoxide, a key mechanism of killing in insect haemocytes and mammalian phagocytes. It showed reduced virulence in the murine yersiniosis infection model and in contrast to the wild-type strain IP32953 was unable to kill G. mellonella. The complemented mutant regained all phenotypic properties associated with SodC, confirming the important role of this metalloenzyme in two Y. pseudotuberculosis infection models.

Correspondence
Richard W. Titball
r.w.titball{at}exeter.ac.uk

Abbreviations: CI, competitive index; i.v., intravenous(ly); SOD, superoxide dismutase

{dagger}Present address: School of Life Sciences, Kingston University, Penrhyn Road, Kingston upon Thames KT1 2EE, UK.






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