Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 155 (2009), 1901-1911; DOI  10.1099/mic.0.026062-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
mic.0.026062-0v1
155/6/1901    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Douillard, F. P.
Right arrow Articles by O'Toole, P. W.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Douillard, F. P.
Right arrow Articles by O'Toole, P. W.
Agricola
Right arrow Articles by Douillard, F. P.
Right arrow Articles by O'Toole, P. W.
Microbiology 155 (2009), 1901-1911; DOI  10.1099/mic.0.026062-0
© 2009 Society for General Microbiology

Effect of FliK mutation on the transcriptional activity of the {sigma}54 sigma factor RpoN in Helicobacter pylori

Francois P. Douillard1, Kieran A. Ryan1, Jason Hinds2 and Paul W. O'Toole1

1 Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Western Road, Cork, Ireland
2 Bacterial Microarray Group, Division of Cellular and Molecular Medicine, St George's University of London, Cranmer Terrace, London SW17 0RE, UK

Helicobacter pylori is a motile Gram-negative bacterium that colonizes and persists in the human gastric mucosa. The flagellum gene regulatory circuitry of H. pylori is unique in many aspects compared with the Salmonella/Escherichia coli paradigms, and some regulatory checkpoints remain unclear. FliK controls the hook length during flagellar assembly. Microarray analysis of a fliK-null mutant revealed increased transcription of genes under the control of the {sigma}54 sigma factor RpoN. This sigma factor has been shown to be responsible for transcription of the class II flagellar genes, including flgE and flaB. No genes higher in the flagellar hierarchy had altered expression, suggesting specific and localized FliK-dependent feedback on the RpoN regulon. FliK thus appears to be involved in three processes: hook-length control, export substrate specificity and control of RpoN transcriptional activity.

Correspondence
Paul W. O'Toole
pwotoole{at}ucc.ie

Abbreviations: CGH, comparative genome hybridization; qRT-PCR, quantitative real-time PCR

The microarray design is available in B µG@Sbase (accession number A-BUGS-18; http://bugs.sgul.ac.uk/A-BUGS-18) and also ArrayExpress (accession number A-BUGS-18). Fully annotated microarray data have been deposited in B µG@Sbase (accession number E-BUGS-78; http://bugs.sgul.ac.uk/E-BUGS-78) and also ArrayExpress (accession number E-BUGS-78).

A supplementary table, listing oligonucleotide primers used in this study, is available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.