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Microbiology 155 (2009), 1934-1939; DOI  10.1099/mic.0.026641-0
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Microbiology 155 (2009), 1934-1939; DOI  10.1099/mic.0.026641-0
© 2009 Society for General Microbiology

Pseudomonas aeruginosa Las quorum sensing autoinducer suppresses growth and biofilm production in Legionella species

Soichiro Kimura1, Kazuhiro Tateda1, Yoshikazu Ishii1, Manabu Horikawa2, Shinichi Miyairi3, Naomasa Gotoh4, Masaji Ishiguro2 and Keizo Yamaguchi1

1 Department of Microbiology and Infectious Diseases, Toho University Faculty of Medicine, Ota-ku, Tokyo 143-8540, Japan
2 Suntory Institute for Bioorganic Research, Mishima-gun, Osaka 618-8503, Japan
3 Laboratory of Bio-organic Chemistry, College of Pharmacy, Nihon University, Funabashi, Chiba 274-8555, Japan
4 Department of Microbiology, Kyoto Pharmaceutical University, Yamashina, Kyoto 607-8414, Japan

Bacteria commonly communicate with each other by a cell-to-cell signalling mechanism known as quorum sensing (QS). Recent studies have shown that the Las QS autoinducer N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) of Pseudomonas aeruginosa performs a variety of functions not only in intraspecies communication, but also in interspecies and interkingdom interactions. In this study, we report the effects of Pseudomonas 3-oxo-C12-HSL on the growth and suppression of virulence factors in other bacterial species that frequently co-exist with Ps. aeruginosa in nature. It was found that 3-oxo-C12-HSL, but not its analogues, suppressed the growth of Legionella pneumophila in a dose-dependent manner. However, 3-oxo-C12-HSL did not exhibit a growth-suppressive effect on Serratia marcescens, Proteus mirabilis, Escherichia coli, Alcaligenes faecalis and Stenotrophomonas maltophilia. A concentration of 50 µM 3-oxo-C12-HSL completely inhibited the growth of L. pneumophila. Additionally, a significant suppression of biofilm formation was demonstrated in L. pneumophila exposed to 3-oxo-C12-HSL. Our results suggest that the Pseudomonas QS autoinducer 3-oxo-C12-HSL exerts both bacteriostatic and virulence factor-suppressive activities on L. pneumophila alone.

Correspondence
Soichiro Kimura
kimsou{at}med.toho-u.ac.jp

Abbreviations: 3-oxo-C12-HSL, N-(3-oxododecanoyl)-L-homoserine lactone; AHL, acylhomoserine lactone; C4-HSL, N-butanoyl-L-homoserine lactone; QS, quorum sensing






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