Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Microbiology 155 (2009), 2168-2181; DOI  10.1099/mic.0.026690-0
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary data
Right arrow All Versions of this Article:
mic.0.026690-0v1
155/7/2168    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Google Scholar
Right arrow Articles by Arafah, S.
Right arrow Articles by Marceau, M.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arafah, S.
Right arrow Articles by Marceau, M.
Agricola
Right arrow Articles by Arafah, S.
Right arrow Articles by Marceau, M.
Microbiology 155 (2009), 2168-2181; DOI  10.1099/mic.0.026690-0
© 2009 Society for General Microbiology

An iron-regulated LysR-type element mediates antimicrobial peptide resistance and virulence in Yersinia pseudotuberculosis

Sonia Arafah1,2,3,4, Marie-Laure Rosso1,2,3,4,{dagger}, Linda Rehaume5, Robert E. W. Hancock5, Michel Simonet1,2,3,4 and Michaël Marceau1,2,3,4

1 Inserm U801, F-59019 Lille, France
2 Université Lille Nord de France, F-59000 Lille, France
3 Institut Pasteur de Lille, F-59019 Lille, France
4 CHULille, F-59000 Lille, France
5 Department of Microbiology and Immunology, Center for Microbial Diseases and Immunity Research, Lower Mall Research Station, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

During the course of its infection of the mammalian digestive tract, the entero-invasive, Gram-negative bacterium Yersinia pseudotuberculosis must overcome various hostile living conditions (notably, iron starvation and the presence of antimicrobial compounds produced in situ). We have previously reported that in vitro bacterial growth during iron deprivation raises resistance to the antimicrobial peptide polymyxin B; here, we show that this phenotype is mediated by a chromosomal gene (YPTB0333) encoding a transcriptional regulator from the LysR family. We determined that the product of YPTB0333 is a pleiotropic regulator which controls (in addition to its own expression) genes encoding the Yfe iron-uptake system and polymyxin B resistance. Lastly, by using a mouse model of oral infection, we demonstrated that YPTB0333 is required for colonization of Peyer's patches and mesenteric lymph nodes by Y. pseudotuberculosis.

Correspondence
Michaël Marceau
michael.marceau{at}ibl.fr

Abbreviations: MFS, major facilitator superfamily

{dagger}Present address: Unité de Prévention et Thérapies Moléculaires des Maladies Humaines, Centre National de Référence de la coqueluche et autres bordetelloses, 28 rue du Dr Roux, F-75724 Paris cedex 15, France.

A supplementary table, listing putative additional YPTB0333-regulated genes, and a supplementary figure, showing promoter analysis of the YPTB0331 and YPTB0333 genes, are available with the online version of this paper.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2009 Society for General Microbiology.