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Microbiology 155 (2009), 2775-2783; DOI  10.1099/mic.0.027623-0
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Microbiology 155 (2009), 2775-2783; DOI  10.1099/mic.0.027623-0
© 2009 Society for General Microbiology

A novel carotenoid 1,2-hydratase (CruF) from two species of the non-photosynthetic bacterium Deinococcus

Zongtao Sun1, Shaochuan Shen1,2, Chao Wang1, Hu Wang1, Yaping Hu1, Jiandong Jiao1, Tingting Ma1, Bing Tian1 and Yuejin Hua1

1 Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 310029 Hangzhou, China
2 State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, 310032 Hangzhou, China

A novel carotenoid 1,2-hydratase (CruF) responsible for the C-1',2' hydration of {gamma}-carotene was identified in the non-photosynthetic bacteria Deinococcus radiodurans R1 and Deinococcus geothermalis DSM 11300. Gene expression and disruption experiments demonstrated that dr0091 and dgeo2309 encode CruF in D. radiodurans and D. geothermalis, respectively. Their homologues were also found in the genomes of cyanobacteria, and exhibited little homology to the hydroxyneurosporene synthase (CrtC) proteins found mainly in photosynthetic bacteria. Phylogenetic analysis showed that CruF homologues form a separate family, which is evolutionarily distant from the known CrtC family.

Correspondence
Bing Tian
tianbing{at}zju.edu.cn
or
Yuejin Hua
yjhua{at}zju.edu.cn

Abbreviations: GGPP, geranylgeranyl disphosphate; ROS, reactive oxygen species

A supplementary table of primers and two supplementary figures are available with the online version of this paper.






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