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Microbiology 155 (2009), 2988-2993; DOI  10.1099/mic.0.029694-0
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Microbiology 155 (2009), 2988-2993; DOI  10.1099/mic.0.029694-0
© 2009 Society for General Microbiology

Streptomyces roseoverticillatus produces two different poly(amino acid)s: lariat-shaped {gamma}-poly(L-glutamic acid) and {epsilon}-poly(L-lysine)

Masanobu Nishikawa and Kei Kobayashi

Research Institute for Biological Sciences Okayama (RIBS Okayama), 7549-1 Yoshikawa, Kibichuo-cho, Kaga-gun, Okayama 716-1241, Japan

The poly(amino acid)s {gamma}-poly(DL-glutamic acid) (gPGA) and {epsilon}-poly(L-lysine) (ePL) are known to be natural linear poly(amino acid)s secreted by Bacillus spp. and Streptomyces spp., respectively. In this study, a Streptomyces strain producing both ePL and gPGA was identified. Mass spectrometry and other analyses revealed that the gPGA is a mixture of oligomers consisting of 10–13 L-glutamic acid residues linked by isopeptide bonds. In contrast to the known Bacillus gPGA, the glutamic acid oligomers have a cyclodehydrated structure in each molecule. We previously reported that the ePL molecules secreted by the same Streptomyces strain disperse only slightly in an agar culture plate, as though they were larger molecules. This phenomenon is explicable by the observed polyion complex formation between the glutamic acid oligomers and ePLs. The glutamic acid oligomers control the ePL's dispersion, which would also affect the spatial distribution of the ePL's antimicrobial activity. Therefore, gene clustering or common use of the gene was presumed for biosynthesis of the two poly(amino acid)s. However, no gene for biosynthesis of the glutamic acid oligomer was found in the neighbouring region of that for ePL biosynthesis, and the glutamic acid oligomer was produced by a mutant in which the ePL biosynthetic gene was inactivated by gene disruption.

Correspondence
Masanobu Nishikawa
mnishikawa{at}bio-ribs.com

Abbreviations: ePL, {epsilon}-poly-(L-lysine); gPGA, {gamma}-poly(DL-glutamic acid); ls-gPGA, lariat-shaped {gamma}-poly(L-glutamic acid); NAC, N-acetyl-L-cysteine; OPA, o-phthalaldehyde

The GenBank/EMBL/DDBJ accession number for the 7267 bp nucleotide sequence containing the ePL biosynthetic gene of S. roseoverticillatus is AB477240.

Two supplementary figures are available with the online version of this paper.






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