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Genome diversity in Fructobacillus, Leuconostoc and Weissella genera through physical and genetic mapping

¹ Instituto Gulbenkian de Ciência;
² National Institute of Health, INSA;
³ BioFIG, Faculdade de Ciências da Universidade de Lisboa

Pulsed-field gel electrophoresis analysis of chromosomal single and double restriction profiles of 17 strains belonging to three "Leuconostocaceae" genera was done, resulting in physical and genetic maps for 3 Fructobacillus, 6 Leuconostoc and 4 Weissella strains. AscI, I-CeuI, NotI and SfiI restriction enzymes were used together with Southern hybridization of selected probes to provide an assessment of genomic organization diversity in different species. Estimated genome sizes varied from 1408 kb to 1547 kb in Fructobacillus, 1644 kb to 2133 kb in Leuconostoc and from 1371 kb to 2197 kb in Weissella. Other genomic characteristics of interest were analysed such as oriC and terC localization and rrn operon organization. This latter seems markedly different in Weissella, both in number and disposition in the chromosome. Comparison of intra- and inter- generic features are discussed in the light of chromosome rearrangements and genomic evolution.

⁴ E-mail: imchelo{at}igc.gulbenkian.pt