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E. coli heat shock proteins, IbpA and IbpB, affect biofilm formation by influencing the level of extracellular indole

¹ Department of Biochemistry, University of Gdansk;
² University of Gdansk

Development of E. coli biofilm requires differential expression of various genes implicated in cell signaling, stress responses, motility, and synthesis of structures responsible for cell attachment. The ibpAB operon belongs to the stress response genes most induced during growth of E. coli biofilm. We demonstrated for the first time that the lack of IbpAB proteins in E. coli cells inhibited the formation of biofilm, although allowed normal planktonic growth. We showed that ibpAB cells experienced endogenous oxidative stress which might result from a decreased catalase activity. The endogenous oxidative stress in ibpAB cells led to increased expression of tryptophanase, an enzyme which catalyzes synthesis of indole. We demonstrated that the formation of ibpAB biofilm was delayed due to the increase in the extracellular concentration of indole, which is known to play a role of a signal molecule, inhibiting biofilm growth.

³ E-mail: lasko{at}biotech.ug.gda.pl