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Supplementary figures for Microbiology Volume 147, Part 8, pages 2203–2214

C. O. Brämer and A. Steinbüchel The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism

Supp Fig I Nucleotide sequence of the prp locus of R. eutropha HF39. Amino acids are shown in the one-letter code. S/D, Shine/Dalgarno sequence; -10/-35, putative sigma-70 promotor sequence; -12/-24, putative sigma-54 promotor sequence. Inverted triangles indicate a Tn5-insertion locus. The Omega symbol indicates insertion of Omega-Km; the boxed sequence with an Omega symbol at each end indicates deletion of 416 bp and insertion of Omega-Km. TGT-N12-ACA and TTGAATTNCAAA-N8-TTTGNAATTCAA are putative upstream activator sequences.

Supp Fig II Alignment of the translational product of prpB. [1] R. eutropha HF39; [2] R. metallidurans CH34; [3] N. meningitidis; [4] P. putida KT2440; [5] P. aeruginosa; [6] V. cholerae; [7] E. coli and [8] S. typhimurium. Amino acid residues with an identity of 100 % are shaded light grey, residues with an identity of 80 % are shaded grey and residues with 60 % identity are shaded black with white letters.

Supp Fig III Heterologous expression of prpB (A) and acnM (B) in recombinant strains of E. coli XL1-Blue. Soluble crude extracts of E. coli (pSK-/prpB, A2), E. coli (pSK-/acnM, B3) and E. coli (pBluescript SK-, A3 and B2) were separated under denaturating conditions by PAGE and stained for proteins.

Supp Fig IV Alignment of the translational product of prpC. [1] R. eutropha HF39; [2] R. metallidurans CH34; [3] N. meningitidis; [4] P. putida KT2440; [5] P. aeruginosa; [6] V. cholerae; [7] E. coli; [8] S. typhimurium and [9] L. pneumophila. Amino acid residues with an identity of 100 % are shaded light grey, residues with an identity of 80 % are shaded grey and residues with 60 % identity are shaded black with white letters.

Supp Fig V Alignment of the translational product of acnM. [1] R. eutropha HF39; [2] R. metallidurans CH34; [3] N. meningitidis; [4] P. putida KT2440; [5] P. aeruginosa; [6] V. cholerae. Amino acid residues with an identity of 100 % are shaded light grey, residues with an identity of 80 % are shaded grey and residues with 60 % identity are shaded black with white letters.

Supp Fig VI Alignment of the ORF5 translational product. [1] R. eutropha HF39; [2] R. metallidurans CH34; [3] N. meningitidis; [4] P. putida KT2440; [5] P. aeruginosa; [6] V. cholerae. Amino acid residues with an identity of 100 % are shaded light grey, residues with an identity of 80 % are shaded grey and residues with 60 % identity are shaded black with white letters.

Supp Fig VII Alignment of the prpD translational product. [1] R. eutropha HF39; [2] P. putida KT2440; [3] P. aeruginosa; [4]: E. coli; [5] S. typhimurium; [6] L. pneumophila; [7] Dictyostelium. Amino acid residues with an identity of 100 % are shaded light grey, residues with an identity of 80 % are shaded grey and residues with 60 % identity are shaded black with white letters.

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