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Fig. 1. Tn5041 variants. The genetic/restriction map of Tn5041A together with the Southern hybridization probes 1 to 5 (described in Methods) are at the top. E, EcoRI; P, PstI; EV, EcoRV; Sc, SacII; orfP, the ORF encoding a porin-like protein; R’, an incomplete merR gene; 
, the minitransposon with terminal inverted repeats closely related to those of Tn5044 and Tn5046 (Kholodii et al., 2000a ; Mindlin et al., 2001 ; ACs Y17691 and Y18360); 1 and 2, the urf-1 (merE) and urf-2 genes. Other Tn5041 determinants are described in Introduction and Results. The thin lines represent unsequenced DNA; sequenced regions are shown by black bars (Tn5041 core sequence type), white boxes (regions of high divergence) and intermittent thick lines (documented by Yurieva et al., 1997 ; ACs Y09209 and Y09210). Shown on the right are the host strains and (in parentheses) the types of transposons. The point mutations are as follows: in position 811, G
A eliminating the Tn5041 SacII site; 952, GA; 1240, deletion of G; 1241, CT; 1265, TC; 1384, CT; 2875, AG eliminating the Tn5041 EcoRV site; 3773, GA eliminating the Tn5041 PstI site. EV-, P- and Sc- are the EcoRV, PstI and SacII mutant sites. The insertions (symbolized by triangles) are located between nt 5439 and 5440 (all introns, INT), 6984 and 6985 (all mer2 cassettes), and 5900 and 5901 (Tn21
In2 transposons). The LS47-4 deletion (
100 bp) shown by // lies between nt 2394 and 3772. The orientation of INT5041 and the homologous segment of INT5041C are shown by arrows. The different sequenced regions are marked by lower-case letters. The examples, each representing identical allelic sequences, are deposited in the database. The set of the assigned ACs is as follows: a, AJ422111–AJ422116 and Y19000; b, AJ422117; c, AJ422126 and AJ422127; d, AJ422128 and AJ422129; e, AJ422223; f, AJ422227; g, AJ422228; h, AJ422229; i, AJ422230; j, AJ422233; k, AJ422231; l, AJ422232; m, AJ426649; n, AJ422130; the 
1-containing region, Y19001. The sequences of the insertions and the adjacent regions are deposited with the database as single entries (the ACs are listed in Fig. 2 legend).
