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Title of supplement

The MspA porin promotes growth and increases antibiotic susceptibility of both Mycobacterium bovis BCG and Mycobacterium tuberculosis, by C. Mailaender, N. Reiling, H. Engelhardt, S. Bossmann, S. Ehlers and M. Niederweis

Microbiology vol. 150, part 4, pp. 853 - 864

Supplementary Fig. S1. Fluorescence microscopy of M. bovis BCG

The viability and aggregation of bacteria in liquid cultures was checked routinely using the LIVE/DEAD kit (Molecular Probes). A sample of the cell suspension was mixed with an equal volume of a fluorescence dye mixture containing equimolar amounts of propidium iodide and SYTO 9 and was incubated in the dark for 1 min. The stained cells were examined using a Zeiss Axioskop2 fluorescence microscope. Pictures were analysed by counting live (green) and dead (red) bacteria. The mspA-expressing M. bovis BCG (A) showed on average 98 % viable and single cells, the wild-type strain (B) 97 %.







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