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Supplementary movies

Dynamic localization of membrane proteins in Bacillus subtilis, by A. S. Johnson, S. van Horck and P. J. Lewis

Microbiology vol. 150, part 9, pp. 2815 - 2824

Movie 1. Movie of 3D reconstruction of AtpA-GFP fusion shown in Fig. 4 of the main text.

Movie 2. Movie of 30 min time-lapse monitoring of AtpA-GFP movement described in the main text and shown in Fig. 5 of the main text. Each frame represents a 1 min time gap.

Fig. S1. Analysis of ATP synthase movement over a short time period. Panels A-C show separate frames from a time-lapse experiment taken 5 s apart. Panel D shows an overlay of the three frames. Panel E shows a linescan through the cell illustrated with the arrow in panel D, with the same colour scheme for 0 s (red), 5 s (green) and 10 s (blue) in all panels. The majority of the peaks overlap at each time point, indicating that there was little movement of ATP synthase over a 10 s period.





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