Non-conservative mutations in mutS alleles of natural hypermutable isolates
Hypermutable Haemophilus influenzae with mutations in mutS are found in cystic
fibrosis sputum , by M. E. Watson Jr, J. L. Burns and A. L. Smith
Microbiology vol. 150, part 9, pp. 2947-2958
Predicted amino acid sequences translated from mutS DNA sequences
of H. influenzae, aligned using CLUSTAL W (http://clustalw.genome.ad.jp/)
are presented in an Acrobat PDF file.
For comparison to a sequence with previous structure and
function studies, the E. coli K12-MG1655 mutS was included
and is indicated by K12 (sequence from TIGR). The R652 (Rd KW20)
mutS sequence is indicated on the top line and each sequence below
that matches the amino acid in R652 is indicated by a period (.); dashes
(-) indicate the absence of matching amino acids in the sequence. The
CLUSTAL program produces a consensus sequence from all of the
input sequences and attempts to indicate the strength of conservation when
an amino acid substitution occurs: when all sequences have identical amino
acids in one position, a star (*) is indicated in the consensus line; when
an amino acid substitution occurs in one or more sequences with a
relatively strong conservation, a colon (:) is indicated; when a
substitution occurs with a relatively weak conservation, a period (.) is
indicated; when a substitution occurs with no conservation, a blank is
indicated. These strengths of conservation are based on positively scoring
groups in the Gonnet Pam250 matrix.
Assistance in the use and interpretation of CLUSTAL programs can be found at http://www-igbmc.u-strasbg.fr/BioInfo/ClustalX/Top.html.
Copyright © 2009 Society for General Microbiology.
