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Supplementary figures

Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) by host cells, by M. Rupnik, S. Pabst, M. Rupnik, C. von Eichel-Streiber, H. Urlaub and H.-D. Söling

Microbiology vol. 151, part 1, pp. 199 - 208

Supplementary figures (PDF files):

Fig. S1. Processing of TcdB with cytosolic and membrane fraction by different cell lines.
Fig. S2. Time course of cleavage reactions - with Cy3-Tcd_B10463 and Cy3-Tcd_B8864.
Fig. S3. Distribution of TcdB₁₀₄₆₃ and its proteolytic fragments in DonWT cells after incubation with the toxin.
Fig. S4. Peptide mass fingerprint analysis of a 60 kDa fragment of TcdB₈₈₆₄.
Fig. S5. Peptide mass fingerprint analysis of a 60 kDa fragment of TcdB₁₀₄₆₃.

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