The fluorene catabolic linear plasmid in Terrabacter sp. strain DBF63 carries the β-ketoadipate pathway genes, pcaRHGBDCFIJ, also found in proteobacteria, by H. Habe, J.-S. Chung, A. Ishida, K. Kasuga, K. Ide, T. Takemura, H. Nojiri, H. Yamane and T. Omori
Microbiology vol. 151, part 11, pp. 3713 - 3722
Supplementary Table.
Annotated ORFs of the analyzed DNA region of pDBF1.[ PDF file ] (297 KB)
Supplementary Fig. S1
Supplementary Fig. 1. (A) RT-PCR analysis of pcaR, pcaHG, pcaDC and pcaFIJ genes. Total RNA from DBF63 cells grown in FN, DF or protocatechuate (PCA) were used as the templates in RT-PCR. Complementary DNA samples were appropriately diluted prior to PCR. Primer sets (Table 2) and PCR conditions are described in the text. M5 and M6 represent DNA molecular mass marker 5 and 6 (Nippon Gene), respectively. Sizes of the standards (bp) are shown to the left of the image. (B) Expression of pcaD gene in DBF63 cells induced by PCA (left panel) and FN (right panel). RNA preparation and quantitative RT-PCR were performed as described in the text. Transcript levels were normalized using 16S rRNA as an internal control. Results from three measurements are shown with error bars. The mean values of the pcaD mRNA levels in the control samples, supplemented with 50 µl ethanol (EtOH; left panel) or dimethylformamide (DMF; right panel), were arbitrarily set at 1.0. The independent experiments were repeated twice, and a similar tendency was observed both times.[ PDF file ] (525 KB)
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