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Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pylori, by C. Belzer, J. Stoof, C. S. Beckwith, E. J. Kuipers, J. G. Kusters and A. H. M. van Vliet

Microbiology vol. 151, part 12, pp. 3989 - 3995

Fig. S1. H. hepaticus does not survive acid shock. H. hepaticus ATCC 51449 was incubated for 30 min in PBS of pH 7.0, pH 5.5 and pH 3.0 in either unsupplemented PBS (grey bars), PBS with 0.5 mM urea (white bars), or PBS with 100 µM Ni Cl₂ (black bars). Asterisks at pH 3.0 indicate that there was no survival of H. hepaticus at this condition. Data points shown are the mean of three independent experiments. [PDF] (11 kb)

Fig. S2. Urease activity is nickel-induced in H. hepaticus. Urease activity of H. hepaticus strain ATCC 51449 (a) and H. pylori strain 26695 (b) was assessed after 24 h of growth at pH 7.0 in unsupplemented BBC medium (black bars) and BBC medium supplemented with NiCl₂ to a final concentration of 100 µM (white bars). Note the difference in the scale of the y-axis. Each bar represents data from three to five independent experiments for each strain; error bars denote standard deviation. Asterisks indicate a significant increase of urease activity in the test condition (100 µM Ni Cl₂) when compared to the standard growth conditions (unsupplemented medium) (P≤0.05, Mann-Whitney U test). [PDF] (12 kb)

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