Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum, by E. Radmacher, K. C. Stansen, G. S. Besra, L. J. Alderwick, W. N. Maughan, G. Hollweg, H. Sahm, V. F. Wendisch and L. Eggeling
Microbiology vol. 151, part 5, pp. 1359 - 1368
Text files showing microarray data for the entire genome analysed are included here. Experiment series 1 contains the data where mRNA was prepared from ethambutol-treated/untreated cells harvested at constant optical density. Experiment series 2 contains the data where mRNA was prepared from ethambutol-treated/untreated cells harvested at different times. The
generation of whole-genome DNA microarrays, synthesis of fluorescently labelled cDNA from total RNA, microarray hybridization, washing and data analysis were performed as described previously (Ishige et al., 2003; Polen & Wendisch 2004).
Experiment series 1
Experiment series 2_0h
Experiment series 2_2h
Experiment series 2_4h
Experiment series 2_6h
Experiment series 2_8h
Experiment series 2_10h
Experiment series 2_15h
Experiment series 2_25h
REFERENCES
Ishige, T., Krause, M., Bott, M., Wendisch, V. F. & Sahm, H. (2003). The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses. J Bacteriol 185, 4519-4529.
Polen, T. & Wendisch, V. F. (2004). Genomewide expression analysis in amino acid-producing bacteria using DNA microarrays. Appl Biochem Biotechnol 118, 215-232.
Copyright © 2009 Society for General Microbiology.