The dynamic behaviour of microtubules and their contributions to hyphal tip growth in Aspergillus nidulans, by K. Sampson and I. B. Heath
Microbiology vol. 151, part 5, pp. 1543 - 1555
Supplementary Fig. S1. Photobleaching of a hyphal tip cell showing no evidence of microtubule (MT) fluxing/treadmilling. Time in seconds in each frame. Time 0 is prior to photobleaching. Time 1 shows the photobleached region (photobleaching between 1 and 60 s). Arrows (large and small) follow the edge of the photobleached region MTs. No translocation of the photobleached or non-photobleached region is detectable within MTs. Times 68 and 74; arrowheads identify an MT elongating into the photobleached region. Time 82; arrowhead points to an MT elongating through the photobleached region. Time 142; fluorescence recovery (photobleached region is no longer distinct from the rest of the cell). Bar, ~2 µm.
Supplementary Fig. S2. Cytoplasmic fluorescence intensity gradients for the region between the tip and the apical nucleus during mitosis (from prophase to the start of aster formation). Positive gradients are tip-low and negative ones tip-high. (a) Four examples of low-MT cells. (b) Five examples of medium-MT cells.
Copyright © 2009 Society for General Microbiology.
