Calcium influences cellular and extracellular product formation during biofilm-associated growth of a marine Pseudoalteromonas sp., by M. A. Patrauchan, S. Sarkisova, K. Sauer and M. J. Franklin
Microbiology vol. 151, part 9, pp. 2885 - 2897
Supplementary Figure
Subsections of two-dimensional gels of Pseudoalteromonas sp. protein extracts showing the effect of biofilm-associated growth and calcium addition on the amounts of individual proteins. Columns 1, 2, 3 and 4 are gel sections of protein extracts from cells cultured in minimal marine medium (MMM) with 0.25, 1.0, 5.0 and 10.0 mM CaCl2. Rows A, C, E and G are gel sections of protein extracts from cells in biofilm culture. Rows B, D, F and H are gel sections of protein extracts from cells in planktonic culture. Row A, B spot 1 – an example of a class I protein – shows an increased amount in biofilms, but is not affected by Ca2+. Rows C, D, spot 3 – an example of a class II protein – shows an increased amount in biofilms, and is influenced by Ca2+. Rows E, F, spots 14 and 15 – examples of class III proteins – show a decreased amount in biofilms, and are unaffected by Ca2+. Rows G, H, spot 16 – an example of a class IV protein – shows a decreased amount in biofilms, but is affected by Ca2+.[ PDF file ] (255 KB)
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