Dual regulation of a polyethylene glycol degradative operon by AraC-type and GalR-type regulators in Sphingopyxis macrogoltabida strain 103, by J. Charoenpanich, A. Tani, N. Moriwaki, K. Kimbara and F. Kawai

Microbiology vol. 152, part 10, pp. 3025 - 3034

Table S1. Bacterial strains and plasmids used in this study. [PDF] (67 kb)

Table S2. Primers used in this study. [PDF] (29 kb)

Fig. S1. Determination of transcription start sites of pegB, pegA and pegR. The nucleotide sequences of the pegB, the pegA and the pegR promoter regions are shown in (a), (b) and (c), respectively. The transcription start sites, which were determined by 5'-RACE, are indicated by the angled arrow for pegB and pegR and the dashed angled arrow for pegA. In (b), the angled arrow shows the pegA transcription start site determined by primer extension analysis (d). The sequence is numbered relative to the transcription start site from 5'-RACE results (the bold G nucleotide). The putative translation start site, RBS, -10 region, -35 promoter sequence, and histone-like protein HU binding site (hbs) are underlined. Boxes indicate the putative consensus transcriptional regulator binding regions. (d) Primer extension analysis of pegA. The products of reverse-transcription (lane 1, PEG-4000-grown cells and lane 2, glucose-grown cells) were coelectrophoresed with a DNA-sequencing ladder (A, G, C, and T) of the pCR-PpegA. The expanded views of the nucleotide sequence around the transcription initiation sites (t1 and t2) are shown. [PDF] (75 kb)

Fig. S2. MALDI-TOF MS spectrum of the trypsin-digested pegA promoter-binding proteins. (a) The 20 kDa protein and (b) The 40 kDa protein. [PDF] (44 kb)

This Article

	Abstract
	Full Text

Services

	Email this article to a friend
	Alert me to new issues of the journal


Citing Articles

	Citing Articles via CrossRef

INT J SYST EVOL MICROBIOL	MICROBIOLOGY	J GEN VIROL
J MED MICROBIOL	ALL SGM JOURNALS