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Bacterial adhesion to and viability on positively charged polymer surfaces, by A. Terada, A. Yuasa, T. Kushimoto, S. Tsuneda, A. Katakai and M. Tamada

Microbiology vol. 152, part 12, pp. 3575 - 3583

Figs S1-S5 (see below for legends). [PDF] (40 kb)

Fig. S1. Degree of GMA grafting as a function of reaction time.

Fig. S2. Relationship between contact angle hysteresis and membrane potential.

Fig. S3. Effect of washing procedure on adhesion behaviour of an E. coli cell. The experiment was conducted by immersing E. coli cells in 0.02X PBS solution without the sheets for 8 h. The numbers in parentheses represent the number of washings with 0.02X PBS. The washing procedure was conducted by centrifugation and resuspension in the PBS solution. The degree of washing did not provide different bacterial adhesion behaviour, indicating that the effects of protein adsorption and cell growth on bacterial adhesion are marginal.

Fig. S4. Leakage of functional group from the grafted sheets. Densities were measured before and after 8 h immersion of each sheet in 0.02X PBS devoid of bacterial suspension. Relative density [density at time 8 h (d) to 0 h (d0)] is shown as the ordinate.

Fig. S5. Dependence of E. coli cell viability on membrane potential of each sheet (0.25 h).

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