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Identification of a novel gene, URE2, that functionally complements a urease-negative clinical strain of Cryptococcus neoformans, by A. Varma, S. Wu, N. Guo, W. Liao, G. Lu, A. Li, Y. Hu, G. Bulmer and K. J. Kwon-Chung

Microbiology vol. 152, part 12, pp. 3723 - 3731

Fig. S1. Protein sequence alignment between the sudA gene of A. nidulans and the URE2 gene of C. neoformans. The third row reflects the consensus protein sequence between the two strains. [PDF] (35 kb)

Fig. S2. Transcriptional analysis of urease (URE1) and URE2 genes by RT-PCR in the wild type strain H99, the urease-negative clinical isolate B-4587, the sud1 disruption strain, and the URE2-complemented strain B-4587C. Oligonucleotides used as primers for the analysis were designed from DNA sequences of the urease gene URE1, the URE2 gene, and the constitutively expressed GPD gene as control. [PDF] (126 kb)







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