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Involvement of Geobacter sulfurreducens SfrAB in acetate metabolism rather than intracellular, respiration-linked Fe(III) citrate reduction, by M. V. Coppi, R. A. O'Neil, C. Leang, F. Kaufmann, B. A. Methé, K. P. Nevin, T. L. Woodard, A. Liu and D. R. Lovley

Microbiology vol. 153, part 10, pp. 3572 - 3585

Table S1. QRT-PCR primers [PDF] (14 kb)

Table S2. Genes that are upregulated in the acetate-adapted SfrAB-null strain during growth in chemostats in acetate:fumarate medium [PDF] (22 kb)

Table S3. Genes that are downregulated in the acetate-adapted SfrAB-null strain during growth in chemostats in acetate:fumarate medium [PDF] (40 kb)

Fig. S1. Growth of the wild-type srain and an independently isolated, acetate-adapted SfrAB-null strain in (A) acetate:fumarate (15 mM:20mM) medium and (B) acetate:Fe(III) (20 mM:55 mM) medium. Inocula (3%) were exponential-phase acetate:fumarate cultures. Data are means and standard deviations of triplicate cultures. PowerPoint file (68 kb)

Fig. S2. Preliminary biochemical characterization of an independently isolated, acetate-adapted SfrAB-null strain. (A) Determination of NADPH-dependent Fe(III)-NTA, AQDS, and benzyl viologen reductase activities in membrane and soluble fractions prepared from the wild-type and SfrAB-null strains. (B) Distribution of the cytoplasmic enzyme, oxoglutarate/ferredoxin oxidoreductase, and the various reductase activities in the membrane and soluble fractions of the wild-type strain. PowerPoint file (50 kb)

Fig. S3. Functional characterization of genes that were differentially expressed in the acetate-adapted SfrAB-null strain during growth in chemostats in acetate:fumarate medium and had a fold change in expression of >1.5 or <-1.5 relative to wild type. Functional categories were created and assigned by the authors based on analysis of protein sequences. The category Unknown includes both hypothetical proteins and conserved proteins of unknown function. PowerPoint file (63 kb)

Overview and additional discussion of the microarray analysis [PDF] (492 kb)

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