HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef

Exploring the evolution of the Bacillus cereus group repeat element bcr1 by comparative genome analysis of closely related strains, by A. Klevan, N. J. Tourasse, F. B. Stabell, A.-B. Kolstø and O. A. Økstad

Microbiology vol. 153, part 11, pp. 3894 - 3908

Table S1. Chromosomal coordinates of 159 full-length bcr1 repeats in the genomes of B. cereus ATCC 10987 (Cereus_10987; 84 full-length repeats; AE017194), B. anthracis Ames (Ba_Ames; 15 full-length repeats; AE016879), B. thuringiensis 97-27 (Bt_Konkukian; 29 full-length repeats; AE017355), B. cereus E33L (formerly known as Zebra Killer, Cereus_ZK; 31 full-length repeats; CP000001). [PDF] (13 kb)

Table S2. Chromosomal coordinates of 493 partial bcr1 repeats in the genomes of B. cereus ATCC 10987 (Cereus_10987; 212 partial repeats; AE017194), B. anthracis Ames (Banthracis_Ames; 91 partial repeats; AE016879), B. thuringiensis 97-27 (Bt_konkukian; 93 partial repeats; AE017355), B. cereus E33L (formerly known as Zebra Killer, Cereus_ZK; 97 partial repeats; CP000001). [PDF] (30 kb)

Fig. S1. Multiple sequence alignment of 159 full-length bcr1 repeats identified in the genomes of B. anthracis Ames, B. thuringiensis subsp. konkukian (97-27), B. cereus E33L and B. cereus ATCC 10987, abbreviated as Ba, Btk, E33L and Bce, respectively. Sequences were aligned using CLUSTAL W (Thompson et al., 1994) followed by manual editing using SEAVIEW (Galtier et al., 1996). A plot of the conservation of each site was generated by CLUSTAL_X (Thompson et al., 1997) and is shown below the alignment. Nucleotides are coloured by the following scheme: dA (red), dC (cyan), dG (orange), dT (green). Sequence lengths are given to the left of the alignment. Indels are represented by dashes. The asterisk at the top of the alignment indicates the only site conserved in all sequences. [PDF] (2.2 Mb)

Fig. S2. Predicted RNA folding, using MFOLD (Zuker, 2003), of the 159 full-length bcr1 repeats identified in the genomes of B. anthracis Ames, B. thuringiensis subsp. konkukian (97-27), B. cereus E33L (Cereus ZK) and B. cereus ATCC 10987, with predicted ΔG (dg) values (kcal mol^-1; 1 kcal=4.184 kJ). [PDF] (2.9 Mb)

REFERENCES
Galtier, N., Gouy, M. & Gautier, C. (1996). SEAVIEW and PHYLO_WIN: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543-548.
Thompson, J. D., Higgins, D. G. & Gibson, T. J. (1994). CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673-4680.
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. (1997). The CLUSTAL_X Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876-4882.
Zuker, M. (2003). Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31, 3406-3415.

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef