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Making sense of quorum sensing in lactobacilli: a special focus on Lactobacillus plantarum WCFS1, by M. H. J. Sturme, C. Francke, R. J. Siezen, W. M. de Vos and M. Kleerebezem

Microbiology vol. 153, part 12, pp. 3939 - 3947

Fig. S1. Bootstrapped neighbour-joining trees showing the phylogenetic relationships of HPK (left) and RR (right) homologues of (putative) QS-TCSs from lactobacilli. Closely related (QS-)TCS from other genera belonging to the Firmicutes (Enterococcus, Staphylococcus, Leuconostoc) were included for improved phylogenetic assignment of some Lactobacillus QS-TCSs. Homologues were collected using iterative BLASTP searches (PSI-BLAST) and aligned using CLUSTAL X. Bootstrap values are shown at the nodes. [PDF] (39 kb)

Fig. S2. Gene context of the various putative QS-TCS in lactobacilli, as obtained from the ERGO database. The agrBDCA QS system of Staphylococcus haemolyticus has been included for comparison. Arrows indicate genes, and gene-context colour-coding is shown in the inset. [PDF] (59 kb)

Fig. S3. Architecture and gene context of two-component histidine protein kinases and response regulators of L. plantarum WCFS1 that contain quorum-sensing-associated motifs and domains according to SMART descriptions (http://smart.embl-heidelberg.de/smart) or PFAM families (http://www.sanger.ac.uk/software/pfam). (A) HPK10-subfamily type HPKs and LytTR-family type RRs. (B) HPK7-subfamily type HPKs and LuxR-family type RRs. HATPase, histidine-kinase-like ATPase-domain; REC, CheY-homologous receiver domain; PFAM LytTR, HTH-LytTr DNA-binding domain; HTH-LuxR, helix-turn-helix Lux regulon; HTH-AraC, helix-turn-helix arabinose operon control protein. Rectangles in HPKs indicate predicted or experimentally determined transmembrane segments (see also Table 1 of the main paper). Below each HPK and RR the genomic organization of the HPK and RR and their flanking regions are shown. The description of relevant genes can be found in the text. Hairpins indicate potential transcription termination sites.

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef