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The role of protein secretion systems in the virulence of the intracellular pathogen Legionella pneumophila

Laboratory of Bacteriology, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium

Correspondence
Jozef Anné
Jozef.anne{at}rega.kuleuven.be

	ABSTRACT

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
Legionella pneumophila is a Gram-negative facultative intracellular pathogen, which multiplies in protozoa in its natural environment and can cause Legionnaires' disease in man, following infection of alveolar macrophages. In each of the different stages of infection of host cells, virulence proteins need to be delivered to their specific place of action and therefore must cross two barriers: the inner and the outer membrane. To date, several specialized secretion machineries for transport of proteins across the inner and outer membrane have been identified in L. pneumophila. Most of these secretion pathways have been shown to affect the virulence of this pathogen. An overview will be given of all the secretion pathways and the proteins transported by these secretion systems identified so far, with special attention paid to those that play a role in the pathogenicity of L. pneumophila.

	Legionella pneumophila

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
Legionella pneumophila is a Gram-negative, rod-shaped facultative intracellular pathogen. It is the causative agent of Legionnaires' disease, a very severe form of pneumonia, and can also cause Pontiac fever, a milder, flu-like, self-limiting disease. Legionellae mainly reside in freshwater environments but also in man-made aquatic systems, which are the major sources of infection. In its natural environment L. pneumophila multiplies primarily within protozoa, but it can also be associated with biofilms. Protozoa provide nutrients, protection in harsh environmental conditions and also make the bacteria more virulent. Infection takes place by inhalation of contaminated aerosols, followed by replication in human alveolar macrophages (reviewed by Albert-Weissenberger et al., 2007).

After internalization into host cells, L. pneumophila resides in a phagosome that does not fuse with the lysosomes of the host cell and then recruits endoplasmic reticulum (ER)-derived vesicles and mitochondria from the host. In this vacuole, the bacteria are converted to a replicative form. When nutrients are depleted, the bacteria enter the transmissive phase and express virulence proteins, resulting in lysis of host cells and the initiation of a new infection round (reviewed by Steinert et al., 2007). In each of these stages of infection, protein secretion is necessary in order to deliver virulence proteins to their specific place of action.

	Protein secretion and its role in virulence

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
To establish a successful pathogen–host interaction, virulence proteins of the pathogen need to be transported to the bacterial surface, to the extracellular environment or directly into the host cell. This is a challenge for Gram-negative bacteria because secreted proteins must cross the inner and outer membrane. For secretion across the inner membrane, proteins with an N-terminal signal peptide are transported by the Sec or Tat secretion machinery. During translocation the signal peptide is cleaved off by the membrane-bound signal peptidase. For extracellular secretion or direct secretion into host cells, various specialized secretion machineries (named type I–V) exist in Gram-negative bacteria, each responsible for the transport of a specific subset of proteins. These are guided to the correct secretion route based on the presence of different secretion signals. Not all Gram-negative bacteria possess all types of secretion systems (reviewed by Gerlach & Hensel, 2007).

Secretion systems in L. pneumophila have been studied extensively because of the essential role of protein secretion in bacterial infection. For transport across the inner membrane the Sec and Tat secretion systems have both been identified in L. pneumophila. A lot of attention has been paid to secretion systems for transport across the outer membrane: the type II PilD-dependent Lsp secretion system, the type IVB Icm/Dot secretion pathway and the type IVA Lvh system. In addition, two putative secretion systems have been identified: the type I Lss secretion machinery and a putative type V secretion system identified in L. pneumophila strain Paris. In this review we will give an overview of recent knowledge regarding these secretion systems, their protein substrates and their possible role in virulence. An overview of the structural proteins and substrates of the main secretion systems of L. pneumophila is given in Table 1. A schematic representation of the main secretion machineries in Gram-negative bacteria that are also present in L. pneumophila is shown in Fig. 1. For details of the different components of these systems the reader is referred to the recent review of Gerlach & Hensel (2007).

View larger version (25K): [in this window] [in a new window]   Fig. 1. Schematic representation of the main secretion systems in Gram-negative bacteria that are also present in L. pneumophila (adapted from Gerlach & Hensel, 2007; for detailed information on the different components of the secretion machineries see this reference). OM, outer membrane; IM, inner membrane; CMhost, cytoplasmic membrane of host cell; OMP, outer-membrane protein; MPF, membrane fusion protein; ABC, ABC transporter. The symbol <$>\raster="rg1"<$> represents a β-barrel pore.

	Protein transport across the inner membrane

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

For transport across the inner membrane, the Sec pathway is generally used. In Escherichia coli the main components of this secretion system are a membrane protein complex (the SecYEG translocase) that forms the secretion pore, a cytoplasmic membrane-associated ATPase (SecA) that provides the energy for transport, and a chaperone (SecB). However, also other proteins such as SecD and SecF and YajC can play a role in Sec secretion (reviewed by Gerlach & Hensel, 2007; see Fig. 1). Many Sec translocase subunits are essential for viability and are conserved in evolution. However, the different sec genes are not present in all bacteria and also the essentiality of these genes is not conserved between organisms (van der Sluis & Driessen, 2006). Inspecting the genome of L. pneumophila Philadelphia, all sec genes except secG could be found (Lammertyn & Anné, 2004). The Legionella Sec pathway has not been further characterized yet.

In addition to the Sec secretion system, the twin-arginine translocation or Tat secretion pathway can be responsible for transport of proteins across the inner membrane. The Tat system, in contrast to the Sec system, allows transport of folded proteins that have the typical characteristic of bearing two arginine residues in their signal peptide. The exact mechanism of Tat transport is not fully determined yet but a complex of TatB and TatC proteins probably recognizes the Tat substrate, followed by transport through a pore consisting of multiple TatA proteins (reviewed by Lee et al., 2006; see Fig. 1).

In L. pneumophila Philadelphia (De Buck et al., 2005) and in L. pneumophila strain 130b (Rossier & Cianciotto, 2005) the tatA, tatB and tatC genes were identified. In L. pneumophila Philadelphia the tat genes are expressed in all growth phases and also when cells grow intracellularly in the host Acanthamoeba castellanii. Tat secretion in this strain was shown to play a role in intracellular replication in amoebae and macrophages, which is a first indication of its role in virulence. Furthermore, it is important for stress response, motility and biofilm formation. Several methods were used to identify putative Tat substrates and proteins that are important for the motility and virulence of L. pneumophila, such as the virulence protein phospholipase C PlcA, which was shown to be Tat dependent (De Buck et al., 2005). Also an inner-membrane protein, the Rieske iron–sulfur protein PetA, was shown to be a Tat substrate. The Rieske iron–sulfur protein is an essential subunit of mitochondrial and bacterial bc₁ complexes, which are central redox carriers in respiratory electron-transport chains, and therefore important for growth and viability. It was concluded that the Tat pathway is necessary for the correct membrane insertion of this protein (De Buck et al., 2007).

For L. pneumophila 130b, the Tat pathway was also shown to play a role in intracellular replication in differentiated U937 cells, but a decrease in intracellular replication in the amoeba Hartmanella vermiformis was observed only under iron-limiting conditions. The secretion of the proteins PlcA and PetA was also experimentally confirmed to be Tat dependent (Rossier & Cianciotto, 2005).

	Protein transport across the outer membrane

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
The putative type I Lss secretion system
A type I secretion system can transport proteins in one step across both membranes and consists of three proteins: an inner-membrane ATPase called the ABC (ATP-binding cassette) transporter, a membrane fusion protein spanning the periplasmic space and an outer-membrane protein (reviewed by Gerlach & Hensel, 2007; see Fig. 1).

In L. pneumophila a putative type I secretion system named Lss has been identified, based on the identification of a homologue of an ABC transporter. Using Southern blots, the distribution of the lss genes was analysed in various Legionella strains and it was suggested that a complete lss gene cluster is restricted to L. pneumophila. Normal expression of the putative lss operon was shown by RT-PCR (Jacobi & Heuner, 2003). A further characterization of this secretion system and its substrates has not been carried out yet and it remains to be determined if this secretion system plays a role in virulence.

The type II PilD-dependent Lsp secretion system
Type II secretion is a two-step process in which transport across the inner membrane is first performed by the Sec or Tat machinery, based on the type of signal peptide of the protein substrates. The proteins then enter the type II machinery, consisting of a multi-subunit complex inserted in the inner membrane and a ring-shaped complex with a central cavity in the outer membrane (reviewed by Gerlach & Hensel, 2007; see Fig. 1).

To date, a type II secretion system in an intracellular pathogen has only been identified in L. pneumophila and Yersinia sp., which can also have an intracellular phase of survival and growth in macrophages (Cianciotto, 2005). The L. pneumophila type II secretion system was first identified based on the presence of PilD, a homologue of the Pseudomonas prepilin peptidase that is necessary for the biogenesis of type IV pili and a functional type II secretion system. A pilD mutant showed a decrease in replication in amoebae and macrophages. This growth defect was attributed to the absence of type II secretion, because the inactivation of type IV pilus biogenesis has no impact on intracellular growth (Liles et al., 1998). The type II secretion apparatus itself is encoded by the lspFGHIJK, lspDE, lspC, lspL and lspM genes (Legionella secretion pathway). lspDE encode the outer-membrane secretin LspD and the ATPase LspE, lspF encodes the inner-membrane protein LspF, lspGHIJK encode the pseudopilins LspGHIJK, and lspC, lspL and lspM encode conserved components of type II secretion pathways. A decrease in replication in both amoebae and macrophages was observed for single lspDE, lspG, lspK and lspF mutants. In addition, the important role of this secretion system in the virulence of L. pneumophila was confirmed because type II secretion was shown to play a role in the multiplication and survival of L. pneumophila in mammalian lungs (Rossier et al., 2004). Both type IV pili and the type II secretion system also play a role in the colonization and establishment of biofilms by L. pneumophila (Lucas et al., 2006).

Several enzymes have been shown to be type II substrates: the major zinc metalloprotease ProA or Msp, an RNase, acid phosphatases, the lipases LipA and LipB, the phospholipase C PlcA, the lysophospholipase A PlaA, the phospholipase A PlaB and the acyltransferase PlaC. None of these type II substrates play a role in intracellular growth in host cells. The proteins ProA, Map acid phosphatase and PlaA were recently also identified in a 2D protein analysis as proteins that were absent or greatly reduced in a type II secretion mutant. Additional differential proteins with a predicted signal peptide were aminopeptidases, an RNase, a chitinase and several proteins with no homology to known proteins. The chitinase was shown to play a role in intracellular replication in the lungs of A/J mice (DebRoy et al., 2006).

Transcription of pilD increases at temperatures below 37 °C and thus the type II secretion system promotes growth at lower temperatures (Liles et al., 1998). Information on the regulation of type II secretion is scarce, but recently it was shown that the global regulators LetA and RpoS regulate the secretion of phospholipase A, lysophospholipase A and acyltransferase. Because no other type II substrates or type II-dependent activities were affected, these are probably not direct regulators of the type II machinery (Broich et al., 2006).

The type IVA Lvh and type IVB Icm/Dot secretion systems
The type IV secretion system was first defined based on the Agrobacterium tumefaciens T-DNA transfer system (Vir system or type IVA system) and the IncP and IncN bacterial conjugation systems (Tra system or type IVB system). The type IV secretion machinery consists of a channel and a pilus for association with the recipient cell, because the type IV system can also transport proteins into the target cells (reviewed by Gerlach & Hensel, 2007; see Fig. 1).

In L. pneumophila a type IVA secretion system named the Lvh (Legionella vir homologues) system is present. This secretion system is encoded by 11 vir homologues (lvhB2, B3, B4, B5, B7, B6, B8, B9, B10, B11, D4), situated in a region with an elevated G+C content, suggesting acquisition from another source (Segal et al., 1999). Interestingly, in L. pneumophila strains Philadelphia, Paris and Lens this lvh region is encoded in a region that either occurs integrated in the chromosome or can be excised as a plasmid-like element (Steinert et al., 2007). In addition to the vir region, five other genes were found in its proximity and named lvr genes (Legionella vir region). The function of these genes is unknown except for LvrC, which is homologous to the E. coli carbon storage regulator CsrA and has been suggested to be a regulator of the expression of the lvh genes. However, there is no experimental evidence for this hypothesis (Segal et al., 1999). The Lvh system is important for efficient host cell infection by L. pneumophila grown at 30 °C, suggesting a role in environmental conditions (Ridenour et al., 2003). Based on PCR experiments it was shown that lvh genes are present in several L. pneumophila patient isolates, in contrast to environmental isolates, suggesting a correlation between the presence of these genes and virulence (Samrakandi et al., 2002).

The Icm (intracellular multiplication)/Dot (defective organelle trafficking) system is a type IVB system and is encoded in two pathogenicity regions containing seven genes (icmV, W and X and dotA, B, C and D) and 18 icm genes (T, S, R, Q, P, O, N, M, L, K, E, G, C, D, J, B, F and H) (reviewed by Segal & Shuman, 1998; Albert-Weissenberger et al., 2007). The specific functions of the Icm/Dot proteins are not known, although some interactions and complexes have been identified. IcmR was identified as a chaperone for IcmQ, which is a pore-forming protein. In addition, the cytosolic components IcmS and IcmW make up a stable complex that facilitates the translocation of substrate proteins. Recently, a subcomplex consisting of five Icm/Dot proteins that bridges the inner and outer membrane was also identified (Vincent et al., 2006b).

Most of the icm/dot genes play a role in intracellular replication of L. pneumophila in amoebae and macrophages and also in guinea pig infection (Segal & Shuman, 1999; Edelstein et al., 1999). The Icm/Dot system is the secretion system of L. pneumophila that is probably the most important for the virulence of this pathogen. The Icm/Dot system plays critical roles in the promotion of phagocytosis, creating a nutrient-rich organelle that escapes the lysosomal degradation pathway, maintaining the integrity of the L. pneumophila phagosome, the induction of apoptosis and lysis of the host cells (Molmeret et al., 2007).

To date, type IVB protein substrates have only been identified for the L. pneumophila Icm/Dot secretion system (Segal et al., 2005). Some Icm/Dot substrates possess a C-terminal translocation signal for substrate recognition. Several substrates show increased expression in the early stationary growth phase, suggesting an important role in the infectious phase of the growth cycle. Substrates that probably play a role in creating a phagosome that evades the lysosomal degradation pathway are VipA and VipD. The substrates RalF, LidA and DrrA more likely play a role in the recruitment of ER vesicles. RalF maintains the host ADP ribosylation factor Arf1 in an active state promoting ER recruitment. LidA plays a role in maintaining bacterial cell integrity and possibly also in recruitment of ER vesicles. DrrA is a guanine nucleotide-exchange factor for the host GTPase Rab1 that regulates the vesicular transport processes. Proteins that might be involved in the prevention of early apoptosis are SdhA and SidF. Substrates playing a role in the last step of the infectious cycle are LepA and LepB, which are functionally involved in the release of Legionella from protozoa after intracellular multiplication. Several other Icm/Dot substrates, such as the Leg and Ceg proteins, have also been identified, but their precise function is not known. For a list of all known Icm/Dot substrates, the reader is referred to Table 1 and to the recent review of Ninio & Roy (2007). The majority of the Icm/Dot substrates have little or no effect on intracellular growth in host cells, probably because of the presence of several paralogues and functional redundancy (Ninio & Roy, 2007).

Little information is available on the regulation of Icm/Dot secretion. In the promoter region of some icm genes a specific binding site was found and the regulatory factors RpoS, RelA and LetA have been shown to exert an effect on the transcription of some icm genes. However, no direct binding between consensus motifs and regulators could be shown, so regulation probably takes place in an indirect manner. Upstream of the icmR gene a binding site for the response regulator CpxR was identified and direct regulation was demonstrated for the first time (Gal-Mor & Segal, 2003). Regulation of Icm/Dot secretion is the result of altered transcription not only of the icm/dot genes, but also of the genes encoding the Icm/Dot substrates. The expression of several Icm/Dot substrate-encoding genes with a conserved regulatory element is controlled by the L. pneumophila response regulator PmrA (Zusman et al., 2007).

Regulation of Icm/Dot secretion is also possible at the protein level. The DotL protein has been suggested to play a role in controlling the opening and closing of the secretion pore. Assembly and/or activation of the Icm/Dot secretion complex would probably also be controlled by several other proteins (Vincent et al., 2006a).

Recently, it was suggested that the Lvh system can functionally replace a defective Icm/Dot system under conditions mimicking aquatic and amoeba-encysted niches of L. pneumophila (Bandyopadhyay et al., 2007).

In addition to the type IVA Lvh and type IVB Icm/Dot system, a new type IVA and several Tra-like (type IVB) systems have been identified in different L. pneumophila strains. The new type IVA secretion system, encoded on a genomic island named Trb-1, was recently identified in the L. pneumophila strain Corby as its genome sequence has now become available. On a 65 kb pathogenicity island that is unique to the Philadelphia strain, a type IVB secretory system, homologous with the Tra proteins of plasmid F of E. coli, was identified (Steinert et al., 2007). A type IVB secretion system was also found in L. pneumophila strain AA100, but not in the Philadelphia strain. This secretion system consists of homologues of the Tra proteins of the R751 plasmid of Enterobacter aerogenes and the RP4 plasmid of E. coli (Samrakandi et al., 2002).

A putative type V secretion system
Type V secretion (or the autotransporter secretion system) is a two-step process in which the C-terminal domain of the substrate forms a β-barrel pore in the outer membrane for translocation of the passenger domain, following Sec-dependent transport across the inner membrane based on the signal peptide (reviewed by Gerlach & Hensel, 2007; see Fig. 1). Based on the identification of a gene encoding an autotransporter in L. pneumophila strain Paris, the presence of a putative type V secretion system has been proposed (Albert-Weissenberger et al., 2007). The functionality and possible substrates of this secretion system have not yet been characterized.

	Conclusion

TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
Now that the genome sequence of several L. pneumophila strains (Philadelphia, Paris and Lens) has been completed, knowledge of the secretion systems of this bacterium is increasing very rapidly. Most secretion systems that are present in other bacterial pathogens have also been identified in L. pneumophila. Interestingly, L. pneumophila is the first and only intracellular pathogen with such an extensively studied type II secretion system. In addition, L. pneumophila is almost the only pathogen with a type IVB secretion system and only for L. pneumophila type IVB substrates have been identified.

It is remarkable that so many different secretion systems could be observed for different L. pneumophila strains. For example, different type IVB secretion systems are present in several L. pneumophila strains. A new type IVA system could only be found for L. pneumophila strain Corby and a type V secretion system could only be identified in L. pneumophila strain Paris. These differences could be explained by the remarkable genome plasticity that has been revealed from comparing the genomes of the sequenced L. pneumophila strains. Genomes of other Legionella strains are being sequenced at the moment and more information on this subject will become available soon.

In the future, it is to be expected that research will mainly focus on the identification of additional substrates of the known secretion systems, their role in the virulence process of L. pneumophila and on the – probably quite complex – regulation of secretion. This information will allow us to better understand the virulence mechanisms of L. pneumophila, which is critical on our way towards the development of novel compounds or strategies to better combat this pathogen.

	ACKNOWLEDGEMENTS

 
E. D. B. is a postdoctoral fellow of FWO. Funding from K.U. Leuven Onderzoeksfonds (PDM/06/245 and OT-project 05/62) is also acknowledged. We regret that we could not cite many important original papers because of space limitations.

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TOP ABSTRACT Legionella pneumophila Protein secretion and its... Protein transport across the... Protein transport across the... Conclusion REFERENCES

 
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