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A new environmentally resistant cell type from Dictyostelium, by I. Serafimidis, G. Bloomfield, J. Skelton, A. Ivens and R. R. Kay

Microbiology vol. 153, part 2, pp. 619 - 630

Movie 1. Aspidocyte formation. Starving cells of strain HM1158 were induced to form aspidocytes in the standard conditions (see Methods of main paper) by 25 µM Cd2+ and 100 nM DIF-1, except that they were plated on coverslips in a chambered microscope slide. Filming started after 5 h of incubation and covers a period of 50 min (1 frame/30 s). [.mov file] (2800 kb)

Movie 2. Aspidocyte dissolution. Aspidocytes were induced from starving cells of strain HM1158 by 25 µM Cd2+ and 100 nM DIF-1 overnight in the standard conditions. They were then washed free of inducers and plated on a coverslip for filming, which started immediately. The film covers a period of 20 min (1 frame per 20 s). [.mov file] (3520 kb)

Table S1. Genes significantly up-regulated in aspidocytes induced by Cd2+/DES or thiabendazole (or in both conditions). [.xls file] (106 kb)

Table S2. Genes significantly down-regulated in aspidocytes by Cd2+/DES or thiabendazole (or in both conditions). [.xls file] (119 kb)
Key to table columns:
A Gene identity at dictyBase (http://dictybase.org)
B. Chromosome
C. Presence of signal peptide
D. Number of transmembrane domains
E. Presence of GPI anchor
F. Pfam domain
G. Additional annotation
H. Induction ratio (log2) with Cd2+/DES compared to un-induced control
I. Induction ratio (log2) with thiabendazole compared to un-induced control
J. Adjusted probability for Cd2+/DES induction
K. Adjusted probability for thiabendazole induction







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