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1 División de Genética, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain
2 Institut für Mikrobiologie und Molekularbiologie, Universität Giessen, Heinrich-Buff-Ring 26-32, D-35392 Giessen, Germany
Correspondence
Asunción Contreras
contrera{at}ua.es
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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), resulting in consumption of the carbon-skeleton of 2-oxoglutarate to yield glutamate. Glutamine synthetase, which catalyses the incorporation of ammonium into glutamate to yield glutamine is the key enzyme of nitrogen assimilation. Two types of glutamine synthetase are produced by the non-diazotrophic cyanobacterium Synechococcus sp. PCC 7942 (hereafter called Synechococcus): GSI, a typical eubacterial glutamine synthetase encoded by glnA, and GSIII, encoded by glnN. The GSIII class is strongly induced in nitrogen-depleted cultures and appears to play a role in acclimation to conditions of nitrogen starvation (Sauer et al., 2000). Due to the lack of 2-oxoglutarate dehydrogenase in cyanobacteria, synthesis of 2-oxoglutarate represents the final step in the oxidative branch of the TCA cycle and directly links 2-oxoglutarate levels to nitrogen assimilation (Herrero et al., 2001). Therefore, the cellular 2-oxoglutarate concentration is an excellent indicator of the cell carbon to nitrogen balance. 2-Oxoglutarate modulates the activity and/or binding properties of three key cyanobacterial nitrogen regulators: the signal transduction protein PII, the transcriptional activator NtcA and the regulatory factor PipX (Forchhammer, 2004; Espinosa et al., 2006).
In cyanobacteria, multiple metabolic and developmental processes are induced by nitrogen starvation. NtcA, the global regulator for nitrogen control, activates genes involved in nitrogen assimilation, heterocyst differentiation and acclimation to nitrogen starvation (Herrero et al., 2001; Luque et al., 2001; Sauer et al., 2000). NtcA belongs to the CAP/CRP (the catabolite activator protein or cyclic AMP receptor protein) family of transcriptional activators. 2-Oxoglutarate stimulates complex formation between PipX and NtcA (Espinosa et al., 2006), binding of NtcA to target sites (Vazquez-Bermudez et al., 2002) and transcription activation in vitro (Tanigawa et al., 2002). NtcA plays a key role in cyanobacterial nitrogen assimilation, being required for the expression of multiple genes repressed by ammonium, the preferred nitrogen source (Herrero et al., 2001). Genes or operons directly regulated by NtcA in Synechococcus include the nirA operon, encoding the nirA-nrtABCD-narB genes for nitrate assimilation, the nirB operon, encoding genes for maximum nitrate assimilation, the glnA and glnN genes for GSI and GSIII, respectively, the glnB for PII, the nblA gene required for phycobilisome degradation (Luque et al., 2001) and the ntcA gene itself. In agreement with this, Synechococcus NtcA-deficient mutants are highly pleiotropic, being unable to assimilate nitrate, upregulate glutamine synthetase (Vega-Palas et al., 1990), or acclimate appropriately to nitrogen starvation (Sauer et al., 1999).
The PII signal transduction protein is one of the most conserved and widespread signal transduction proteins in nature and plays key roles in nitrogen-assimilatory processes (Arcondeguy et al., 2001; Ninfa & Jiang, 2005). Signal transduction by PII (glnB product) in cyanobacteria displays unique features with respect to both covalent modification and downstream signalling (Forchhammer, 2004). Physiological studies showed that PII mediates the short-term ammonium inhibition of nitrate transport (Lee et al., 2000) and controls nitrate uptake in response to light availability (Kloft & Forchhammer, 2005). Furthermore, nitrate reductase in Synechococcus may be directly regulated by PII (Takatani et al., 2006). However, in these cases, no physical interaction with PII was reported. Using yeast two-hybrid approaches, three cyanobacterial PII receptors could recently be identified: the enzyme N-acetylglutamate kinase (NAGK) (Burillo et al., 2004; Heinrich et al., 2004) and the regulatory factor PipX (Burillo et al., 2004; Espinosa et al., 2006) in Synechococcus, and the non-conserved membrane protein PamA in Synechocystis sp. PCC 6803 (Osanai et al., 2005). The physiological role of the PII interaction with these protein receptors is only understood in the case of NAGK. The PII–NAGK regulatory interaction has been conserved across domains of life during the evolution of oxygenic photosynthetic organisms (Burillo et al., 2004; Chen et al., 2006; Sugiyama et al., 2004). PII stimulates NAGK activity and relieves arginine inhibition to different extents in Synechococcus and Arabidopsis thaliana (Chen et al., 2006; Heinrich et al., 2004; Maheswaran et al., 2004).
Binding of PipX to PII and NtcA is inversely affected by 2-oxoglutarate (Espinosa et al., 2006). Complex formation between PipX and PII was impaired by 2-oxoglutarate in the presence of ATP, while the binding of PipX to NtcA could only be observed in the presence of 2-oxoglutarate. In vivo, PipX activated NtcA-dependent promoters glnB and glnN under conditions of nitrogen deficiency, corresponding to high intracellular levels of 2-oxoglutarate, supporting a role for PipX in the activation of NtcA-dependent promoters under nitrogen starvation. In order to investigate the involvement of PipX in nitrogen assimilation and acclimation to nitrogen deficiency, we now analyse the impact of the genetic inactivation of pipX on previously characterized NtcA-controlled processes.
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METHODS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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. Cloning procedures were carried out with Escherichia coli DH5
, using standard techniques. Synechococcus strains were grown photoautotrophically at 30 °C while shaking under constant illumination (75 µE m–2 s–1) provided by cool white fluorescent lights in BG11 medium (nitrate-containing), BG110 (no added nitrogen) or BG11-NH4 (BG110 supplemented with 10 mM NH4Cl, 10 mM HEPES/NaOH pH 7.8). For growth on plates, the medium was solidified by addition of 1 % (w/v) agar. Plates were incubated at 30 °C under constant illumination. For initiation of nitrogen deprivation, mid-exponential cultures (OD750 0.5) were harvested by centrifugation, washed twice with BG110 and finally resuspended in BG110. Whenever appropriate, cultures contained kanamycin (10 µg ml–1) or chloramphenicol (5 µg ml–1).
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). Transformants were selected on chloramphenicol-containing BG11 or BG11-NH4 plates.
Enzymic activities.
Nitrate uptake activity was assayed by measuring nitrate depletion from the external medium as described previously (Lee et al., 1998). BG11 cultures grown to mid-exponential phase were used to determine nitrate and nitrite reductase activities at 30 °C using dithionite-reduced methyl viologen as the electron donor. Nitrate reductase activity (Herrero et al., 1985) was measured in permeabilized cells by including mixed alkyltrimethylammonium bromide (MTA) in the reaction mixture at a final concentration of 50 µg ml–1. Nitrite reductase activity (Herrero & Guerrero, 1986) was also assayed in cells made permeable with MTA (250 µg ml–1). Production (nitrate reductase) or disappearance (nitrite reductase) of nitrite during the reactions was measured as described previously (Snell & Snell, 1949). One unit of enzymic activity corresponded, respectively, to 1 µmol nitrite formed or disappeared per min.
Glutamine synthetase activity (transferase assay) from mid-exponential cultures was measured in permeabilized cells as described by Bender et al. (1977).
Determination of chlorophyll a.
Chlorophyll a concentration was determined from methanolic extracts as described by Mackinney (1941).
Determination of luciferase activity.
To determine bioluminescence, 1 ml of cultures was adjusted with fresh medium to an OD750 of 0.5, supplemented with decanal to a final concentration of 0.25 mM from a 50 mM stock solution made up in 10 % dimethyl sulfoxide. Light emission was recorded in a Berthold LB9509 luminometer. Bioluminescence was recorded every 20 s for 10 min. Light emission increased to a maximum and then declined. Maximum luminescence at the peak, presented as RLU (relative light units) by the instrument, is the value used at each selected time point.
Determination of pigment contents spectrophotometrically.
Wild-type and PipX– strains were grown in BG11 or BG11-NH4 until they reached mid-exponential phase (OD750 0.4–0.5) and then cultures were centrifuged, washed and finally resuspended in BG110. A whole-cell absorbance spectrum was carried out in order to estimate pigment contents. One millilitre of culture was taken at the indicated times, diluted with fresh medium to an OD750 of 0.5 and absorbance spectra (550–750 nm) was recorded on a UV/Visible Ultrospec 3100 pro (Amersham). Pigment content was calculated based on absorbance maxima at 631 nm for phycocyanin and 684 nm for chlorophyll a.
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RESULTS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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). In addition to the NtcA-dependent induction in response to the absence of ammonium, the nirA operon is also subject to activation by the nitrite-dependent activator NtcB (Aichi et al., 2004; Maeda et al., 1998). The fact that the PipX– strain grows well in nitrate-containing medium, where it was originally selected, appeared at odds with our previous suggestions of PipX cooperating with NtcA-dependent activation of target promoters (Espinosa et al., 2006). To investigate the involvement of PipX in the regulation of the nitrate-assimilation genes, we analysed the functions encoded by the nirA operon in a PipX-deficient mutant.
Nitrate and nitrite activities were assayed in cells from the wild-type and PipX– strains adapted to nitrate. As shown in Fig. 1, nitrate and nitrite reductase activities were significantly reduced in the PipX– mutant, thus supporting the implication of PipX in positive regulation of the narB and nirA genes. Nitrate consumption by PipX– strains grown in nitrate-containing media was significantly slower than nitrate consumption by the wild-type strain (compare Fig. 2a, b), thus indicating that PipX deficiency has a negative effect on nitrate assimilation. As is the case with wild-type cells, addition of ammonium to PipX– cells caused an immediate inhibition of nitrate utilization (Fig. 2a, b), indicating that post-translational regulation of nitrate assimilation by PII does not require PipX.
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, the PipX– culture removed nitrate from the medium at a rate significantly lower than the wild-type strain, thus strongly suggesting that PipX has a role on the induction of the nirA operon. Taken together, the results indicate that the Synechococcus PipX– mutant assimilates nitrate with less efficiency than its wild-type counterpart. The defect is more pronounced in cultures previously grown with ammonium, suggesting that PipX is required for rapid acclimation to ammonium withdrawal. On the other hand, the finding that the PipX– mutant retains the ability to tune down nitrate uptake efficiently when the nitrate-adapted cultures are suddenly faced with ammonium suggests that PipX is not required for the regulation of PII functions under conditions of nitrogen sufficiency (low levels of 2-oxoglutarate).
Glutamine synthetase activity is positively regulated by PipX
Nitrogen assimilation by Synechococcus cells requires glutamine synthetase activity, provided mainly by GSI, and under nitrogen-deficiency conditions, also by GSIII. To determine the impact of pipX inactivation on global glutamine synthetase activity, we compared the ability of wild-type and PipX-deficient strains of Synechococcus to induce glutamine synthetase activity upon transfer of cultures from ammonium- or nitrate-supplemented media to media lacking a combined nitrogen source. Comparison of wild-type and PipX– strains indicated that PipX is required for appropriated induction of glutamine synthetase activity (Fig. 3). Glutamine synthetase levels were significantly lower in the mutant strain, an effect observed when the nitrogen source of cultures was either nitrate (compare time 0 in Fig. 3a) or ammonium (compare time 0 in Fig. 3b). In addition, no induction of glutamine synthetase activity was observed in the PipX-deficient strain when cultures were deprived of nitrogen, thus indicating an important deficiency of glutamine synthetase activity in the PipX– mutant. The basal levels of glutamine synthetase activity found in the PipX– strain were indistinguishable from those of the NtcA– strain grown with ammonium or in cells starved of nitrogen for up to 2 days (data not shown).
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PipX is involved in induction of chlorosis but not in the recovery of cultures from nitrogen starvation
When Synechococcus cultures are subjected to nutrient limitation and other stress conditions, they modify the composition of the photosynthetic machinery by degrading their light-harvesting antennae, the phycobilisomes (Schwarz & Forchhammer, 2005). The loss of phycobilisomes and reduction of the chlorophyll a content are responsible for the yellow appearance of the chlorotic cultures. This acclimation process, known as chlorosis or bleaching, requires strong expression of the nblA gene, which is subject to positive control by NtcA (Luque et al., 2001) and NblR, a response regulator involved in general acclimation (Schwarz & Grossman, 1998). NblR deficiency results in a strong non-bleaching phenotype, but the NtcA-deficient mutant is still able to enter chlorosis although it shows a delay in phycobilisome degradation and a faster reduction of the chlorophyll a content (Sauer et al., 1999).
As shown in Fig. 4(a), nitrogen-deprived cultures of the PipX null mutant showed the same phenotype as the NtcA– strain, a weak and transient non-bleaching appearence. Pigment analysis revealed the same pattern of pigment degradation that has been reported for the NtcA strain under nitrogen starvation, a delay in phycocyanin degradation and a faster loss of chlorophyll a content (Fig. 4b–d). As expected for a protein specifically involved in 2-oxoglutarate signalling, induction of chlorosis by sulfur starvation was not affected in the PipX– strain (data not shown).
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). To determine the involvement of PipX in cell survival after chlorosis, wild-type, NtcA– and PipX–mutant strains were tested for their ability to survive for up to 9 days in nitrogen-depleted media. In contrast with the NtcA– strain, the ability of the PipX– strain to resume growth was very similar to that of the wild-type strain (Fig. 5), indicating that PipX is not required for the NtcA-dependent survival of nitrogen-starved cultures.
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). It also includes a recently reported putative light-responsive element (HLR1) involved in negative regulation (Kappell et al., 2006).
Reporter expression was determined by bioluminescence measurements from cultures of wild-type, NtcA– and PipX– mutant derivatives grown with ammonium and shifted to nitrogen-depleted medium. As shown in Fig. 6, the nitrogen starvation response was impaired in both the NtcA– and PipX– mutants but the extent of the impairment was different. Consistent with previous results using luxAB fusions to other NtcA-dependent promoters (Espinosa et al., 2006), the contribution of PipX to nblA upregulation was very significant, but smaller than that of NtcA, indicating that PipX is also involved in the NtcA-dependent transcriptional induction of the nblA gene.
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DISCUSSION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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and data not shown) and induction of chlorosis (Fig. 4a and data not shown) gave similar results with the two mutant strains. Nitrate assimilation, an ability lost in the NtcA– strain, was impaired but still operative in the PipX-deficient mutant (Fig. 2). Given that, at promoters strictly dependent on NtcA for activation, PipX plays a positive but non-essential role for transcription (Espinosa et al., 2006), it is not surprising that PipX– mutants display a less severe or leaky NtcA– phenotype. The auxiliary role of PipX at NtcA-dependent promoters can also be inferred from induction experiments with the PnblA : : luxAB reporter fusion (Fig. 6). The nitrogen-specific role of PipX in chlorosis strongly suggests that PipX participates in the NtcA-dependent activation of the nblA gene. The finding that PipX is not required for survival of cultures after nitrogen starvation (Fig. 5) also supports the view that PipX is involved in the early induction, but not in the long-time maintenance, of NtcA activity.
Taken together, the results presented here strongly suggest that, in addition to the previously analysed glnB and glnN genes (Espinosa et al., 2006) and the nblA promoter studied here (Fig. 6), operons or genes for nitrate assimilation (nirA and probably nirB), glutamine synthesis (glnA) and additional genes for pigment regulation are also under PipX control. Thus, the relatively small but representative sample of NtcA-dependent genes analysed so far at the level of transcriptional induction and/or protein activity demonstrates the importance of PipX in global nitrogen regulation. The mechanism and molecular details involved in transcriptional activation by PipX at NtcA-dependent promoters is presently being addressed.
In vivo, PipX–NtcA and PipX–PII complexes are likely to form under high and low intracellular levels of 2-oxoglutarate, respectively. Consistent with this, the genetic analyses performed so far show the implication of PipX in NtcA activation when the intracellular 2-oxoglutarate levels rise as a consequence of depletion of combined nitrogen from cultures. On the other hand, complex formation between PipX and the PII protein should be expected when cultures are faced with ammonium. Since the PII-dependent inhibition of the nitrate uptake exerted by ammonium remained functional in the PipX– strain (Fig. 2b), the PipX protein does not play a role in the PII-mediated regulation of NRT. Considering that Synechococcus PII proteins are very abundant, able to interact with different partners, and sense and adopt different conformations and modification status according to the nitrogen/carbon ratio, it seems more likely that PipX–PII complexes serve to modulate PipX availability and/or activity. In other words, although the physiological significance of the PipX–PII complexes remains to be established, the experimental evidence gathered so far favours the idea of PipX being a PII target rather than a PII regulator.
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ACKNOWLEDGEMENTS |
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Edited by: R. van Spanning
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REFERENCES |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTSDISCUSSIONREFERENCES |
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Andersson, C. R., Tsinoremas, N. F., Shelton, J., Lebedeva, N. V., Yarrow, J., Min, H. & Golden, S. S. (2000). Application of bioluminescence to the study of circadian rhythms in cyanobacteria. Methods Enzymol 305, 527–542.[Medline]
Arcondeguy, T., Jack, R. & Merrick, M. (2001). P(II) signal transduction proteins, pivotal players in microbial nitrogen control. Microbiol Mol Biol Rev 65, 80–105.
Bender, R. A., Janssen, K. A., Resnick, A. D., Blumenberg, M., Foor, F. & Magasanik, B. (1977). Biochemical parameters of glutamine synthetase from Klebsiella aerogenes. J Bacteriol 129, 1001–1009.
Burillo, S., Luque, I., Fuentes, I. & Contreras, A. (2004). Interactions between the nitrogen signal transduction protein PII and N-acetyl glutamate kinase in organisms that perform oxygenic photosynthesis. J Bacteriol 186, 3346–3354.
Chen, Y. M., Ferrar, T. S., Lohmeir-Vogel, E., Morrice, N., Mizuno, Y., Berenger, B., Ng, K. K., Muench, D. G. & Moorhead, G. B. (2006). The PII signal transduction protein of Arabidopsis thaliana forms an arginine-regulated complex with plastid N-acetyl glutamate kinase. J Biol Chem 281, 5726–5733.
Elhai, J., Vepritskiy, A., Muro-Pastor, A. M., Flores, E. & Wolk, C. P. (1997). Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120. J Bacteriol 179, 1998–2005.
Espinosa, J., Forchhammer, K., Burillo, S. & Contreras, A. (2006). Interaction network in cyanobacterial nitrogen regulation: PipX, a protein that interacts in a 2-oxoglutarate dependent manner with PII and NtcA. Mol Microbiol 61, 457–469.[CrossRef][Medline]
Figurski, D. H. & Helinski, D. R. (1979). Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A 76, 1648–1652.
Forchhammer, K. (2004). Global carbon/nitrogen control by PII signal transduction in cyanobacteria: from signals to targets. FEMS Microbiol Rev 28, 319–333.[CrossRef][Medline]
Hanahan, D. (1985). Techniques for transformation of Escherichia coli. In DNA Cloning, pp. 109–135. Edited by D. M. Glover. Oxford, UK: IRL Press.
Heinrich, A., Maheswaran, M., Ruppert, U. & Forchhammer, K. (2004). The Synechococcus elongatus PII signal transduction protein controls arginine synthesis by complex formation with N-acetyl-L-glutamate kinase. Mol Microbiol 52, 1303–1314.[CrossRef][Medline]
Herrero, A. & Guerrero, M. G. (1986). Regulation of nitrite reductase in the cyanobacterium Anacystis nidulans. J Gen Microbiol 132, 2463–2468.
Herrero, A., Flores, E. & Guerrero, M. G. (1985). Regulation of nitrate reductase cellular levels in the cyanobacteria Anabaena variabilis and Synechocystis sp. FEMS Microbiol Lett 26, 21–25.[CrossRef]
Herrero, A., Muro-Pastor, A. M. & Flores, E. (2001). Nitrogen control in cyanobacteria. J Bacteriol 183, 411–425.
Kappell, A. D., Bhaya, D. & van Waasbergen, L. G. (2006). Negative control of the high light-inducible hliA gene and implications for the activities of the NblS sensor kinase in the cyanobacterium Synechococcus elongatus strain PCC 7942. Arch Microbiol 186, 403–413.[CrossRef][Medline]
Kloft, N. & Forchhammer, K. (2005). Signal transduction protein PII phosphatase PphA is required for light-dependent control of nitrate utilization in Synechocystis sp. strain PCC 6803. J Bacteriol 187, 6683–6690.
Lee, H. M., Flores, E., Herrero, A., Houmard, J. & Tandeau de Marsac, N. (1998). A role for the signal transduction protein PII in the control of nitrate/nitrite uptake in a cyanobacterium. FEBS Lett 427, 291–295.[CrossRef][Medline]
Lee, H. M., Flores, E., Forchhammer, K., Herrero, A. & Tandeau De Marsac, N. (2000). Phosphorylation of the signal transducer PII protein and an additional effector are required for the PII-mediated regulation of nitrate and nitrite uptake in the cyanobacterium Synechococcus sp. PCC 7942. Eur J Biochem 267, 591–600.[Medline]
Luque, I., Zabulon, G., Contreras, A. & Houmard, J. (2001). Convergence of two global transcriptional regulators on nitrogen induction of the stress-acclimation gene nblA in the cyanobacterium Synechococcus sp. PCC 7942. Mol Microbiol 41, 937–947.[CrossRef][Medline]
Mackinney, G. (1941). Absorption of light by chlorophyll solutions. J Biol Chem 140, 315–322.
Maeda, S., Kawaguchi, Y., Ohe, T. A. & Omata, T. (1998). cis-acting sequences required for NtcB-dependent, nitrite-responsive positive regulation of the nitrate assimilation operon in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol 180, 4080–4088.
Maheswaran, M., Urbanke, C. & Forchhammer, K. (2004). Complex formation and catalytic activation by the PII signaling protein of N-acetyl-L-glutamate kinase from Synechococcus elongatus strain PCC 7942. J Biol Chem 279, 55202–55210.
Muro-Pastor, M. I., Reyes, J. C. & Florencio, F. J. (2005). Ammonium assimilation in cyanobacteria. Photosynth Res 83, 135–150.[CrossRef][Medline]
Ninfa, A. J. & Jiang, P. (2005). PII signal transduction proteins: sensors of alpha-ketoglutarate that regulate nitrogen metabolism. Curr Opin Microbiol 8, 168–173.[CrossRef][Medline]
Osanai, T., Sato, S., Tabata, S. & Tanaka, K. (2005). Identification of PamA as a PII-binding membrane protein important in nitrogen-related and sugar-catabolic gene expression in Synechocystis sp. PCC 6803. J Biol Chem 280, 34684–34690.
Sauer, J., Gorl, M. & Forchhammer, K. (1999). Nitrogen starvation in Synechococcus PCC 7942: involvement of glutamine synthetase and NtcA in phycobiliprotein degradation and survival. Arch Microbiol 172, 247–255.[CrossRef][Medline]
Sauer, J., Dirmeier, U. & Forchhammer, K. (2000). The Synechococcus strain PCC 7942 glnN product (glutamine synthetase III) helps recovery from prolonged nitrogen chlorosis. J Bacteriol 182, 5615–5619.
Schwarz, R. & Forchhammer, K. (2005). Acclimation of unicellular cyanobacteria to macronutrient deficiency: emergence of a complex network of cellular responses. Microbiology 151, 2503–2514.
Schwarz, R. & Grossman, A. R. (1998). A response regulator of cyanobacteria integrates diverse environmental signals and is critical for survival under extreme conditions. Proc Natl Acad Sci U S A 95, 11008–11013.
Snell, F. D. & Snell, C. T. (1949). Colorimetric Methods of Analysis, pp. 804–805. New York: Van Nostrand.
Sugiyama, K., Hayakawa, T., Kudo, T., Ito, T. & Yamaya, T. (2004). Interaction of N-acetylglutamate kinase with a PII-like protein in rice. Plant Cell Physiol 45, 1768–1778.
Takatani, N., Kobayashi, M., Maeda, S. & Omata, T. (2006). Regulation of nitrate reductase by non-modifiable derivatives of PII in the cells of Synechococcus elongatus strain PCC 7942. Plant Cell Physiol 47, 1182–1186.
Tanigawa, R., Shirokane, M., Maeda Si, S., Omata, T., Tanaka, K. & Takahashi, H. (2002). Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc Natl Acad Sci U S A 99, 4251–4255.
Vazquez-Bermudez, M. F., Herrero, A. & Flores, E. (2002). 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett 512, 71–74.[CrossRef][Medline]
Vega-Palas, M. A., Madueno, F., Herrero, A. & Flores, E. (1990). Identification and cloning of a regulatory gene for nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942. J Bacteriol 172, 643–647.
Received 19 October 2006;
revised 23 November 2006;
accepted 23 November 2006.
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) or without (
) addition of ammonium. Mean values and standard deviations from four independent assays are presented. (c) Induction of nitrate uptake from ammonium-repressed cultures. Nitrate taken up was measured during 60 min on previously ammonium-grown cells, which were incubated in ammonium-free, nitrate-supplemented BG11 medium for 3 h. Mean values and standard deviations from four independent assays are represented. 
, PipX null mutant; 
, NtcA null mutant.
, PipX null mutant.
