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Fingerprinting fission yeast: polymorphic markers for molecular genetic analysis of Schizosaccharomyces pombe strains, by A.-M. Patch and S. J. Aves

Microbiology vol. 153, part 3, pp. 887 - 897

Fig. S1. Alignment of sequenced PCR products for tandem repeat marker locus within the protein coding sequence of rpb1 for isolates 975 and CBS356. The sequence for 975 h⁺ is identical to the published 972 h^- sequence (Wood et al., 2002). The 21 bp repeated units are indicated by arrows. [PDF] (14 kb)

Fig. S2. Alignment of sequenced PCR products for tandem repeat marker locus within the protein coding sequence of SPCC330.04c for isolates 972 h^-, CBS356 and CBS2775. The sequence for 972 h^- is identical to that of the published sequence (Wood et al., 2002). The 21 bp and 33 bp repeated units are indicated by arrows. [PDF] (14 kb)

Fig. S3. Polymorphism of tif471 minisatellite tandem repeat locus in S. pombe laboratory strains. (a) PCR amplification products of tandem repeat locus within the protein coding sequence of tif471 analysed by agarose gel electrophoresis for laboratory strains: lane 1, 972 h^-; lane 2, 975 h⁺; lane 3, SJA148; lane 4, SJA167; lane 5, SJA122; lane 6, SJA272; lane 7, SJA237; lane 8, SJA175; lane 9, SJA277; lane 10, SJA244; lane 11, SJA216; lane 12, SJA273; lane 13, SJA120; lane 14, SJA238; lane 15, SJA300; lane 16, SJA302. (b) Alignment of the published 972 h^- sequence (Wood et al., 2002) with sequenced PCR products for strains 975 h⁺ and SJA302. The 24 bp repeated units are indicated by arrows. [PDF] (34 kb)

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