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1 Rheinische Friedrich-Wilhelms-Universität Bonn, Institut für Mikrobiologie & Biotechnologie, Meckenheimer Allee 168, 53115 Bonn, Germany
2 Hochschule Niederrhein, Mikrobiologie und Lebensmittelhygiene, Rheydter Straße 277, 41065 Mönchengladbach, Germany
3 Rheinische Friedrich-Wilhelms-Universität Bonn, Physikalisches Institut, Nußallee 12, 53115 Bonn, Germany
4 Louisiana State University, The John Bennett Johnston, Sr. Center for Advanced Microstructures and Devices (CAMD), 6980 Jefferson Highway, Baton Rouge, LA 70806, USA
Correspondence
Alexander Prange
A.Prange{at}gmx.de
Christiane Dahl
ChDahl{at}uni-bonn.de
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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; Pfennig & Trüper, 1974). Although this step is very important in the global sulfur cycle (e.g. Brüser et al., 2000), the process of mobilization and the utilization of different sulfur species is barely understood.
The formal valency of elemental sulfur is zero (S0). However, elemental sulfur tends to catenate and to form chains of various lengths (S
or Sµ) and ring sizes (Sn) (Steudel, 2000; Steudel & Eckert, 2003). Cyclic, orthorhombic 
-sulfur (-S8) (cyclo-octasulfur or S8 rings) is the thermodynamically most stable form of elemental sulfur at ambient temperature and pressure (e.g. Roy & Trudinger, 1970; Steudel, 2000). Accordingly, cyclo-octasulfur is one of the main components of typical commercially available elemental sulfur (‘flowers of sulfur’). In addition, elemental sulfur (flowers of sulfur) always contains some polymeric sulfur consisting of chain-like macromolecules, but the additional presence of large rings, Sn (n>50), is very likely (Steudel & Eckert, 2003). The bonding energy of the S–S bonds in polymeric sulfur is 2.4 kJ mol–1 weaker than that in cyclo-octasulfur (Steudel et al., 1985; Steudel, 1996; Steudel & Eckert, 2003). Accordingly, polymeric sulfur, i.e. chain-like sulfur, might be more easily accessible to sulfur-oxidizing bacteria and for elemental sulfur uptake.
Some studies of the use of solid elemental sulfur by different thiobacilli have been published in recent years (e.g. Silver, 1970; Baldensperger et al., 1974; Espejo & Romero, 1987; Pronk et al., 1992). However, only one study, that of Laishley et al. (1986), has considered the speciation of the elemental sulfur. Those authors investigated the effect of the molecular composition of different forms of elemental sulfur (pure Sµ, pure S8, and a mixture of both and another sulfur species, Sx) on the oxidation of sulfur by Acidithiobacillus albertensis (formerly Thiobacillus albertis; Kelly & Wood, 2000) under aerobic conditions. Those authors suggest that oxidation of elemental sulfur is more dependent on the crystal microstructure than on the specific molecular composition. However, chemical methods were used to produce and determine different elemental sulfur species, and the methods applied (heating, extraction) might have influenced the chemical structure.
During the oxidation of reduced inorganic sulfur compounds (sulfide, thiosulfate) by Alc. vinosum to the final product sulfate, sulfur globules are accumulated as an intermediate inside the cells (Brune, 1995; Dahl & Prange, 2006). The chemical nature of these globules has been determined by Prange et al. (2002a) as sulfur chains (most probably terminated by organic residues at one or both ends) by X-ray absorption near-edge structure (XANES) spectroscopy. This is also an excellent in situ technique to determine the sulfur speciation in biological samples (details can be found in e.g. Prange & Modrow, 2002). The method is non-destructive and a ‘quantitative analysis' of XANES spectra can be performed (e.g. Modrow et al., 2001; Prange et al., 2002a, 2005). Furthermore, XANES can distinguish between sulfur rings and chain structures of zero valent sulfur (Prange et al., 2006).
While Prange et al. (2002a) investigated the speciation of sulfur globules of Alc. vinosum and other sulfur oxidizers, we focused in this study on the question which sulfur species is preferred when Alc. vinosum takes up elemental sulfur. Furthermore, the aim was to analyse the sulfur speciation in the globules of Alc. vinosum after uptake of elemental sulfur and to investigate whether direct cell–sulfur contact is necessary for this process.
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METHODS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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). Solid elemental sulfur (S0; 50 mM) was added as the sole sulfur source to the culture. To maintain pH 7.0±0.1, sterile HCl and Na2CO3 solutions were added by a pH-stat. As an inoculum for the growth experiments, cells of Alc. vinosum grown photoorganoheterotrophically on malate were used and added to the 0 medium. Protein content was determined using the Bradford assay (see below; Bradford, 1976). Two different commercially available forms of elemental sulfur were purchased and used as received from Riedel de Haen (sulfur 1; S1) and from Merck (sulfur 2; S2), respectively, in the fermenter experiments. Furthermore, small amounts of pure polymeric sulfur (50 mM), which were kindly provided by Professor Dr R. Steudel, TU Berlin, were used for culture volumes of 100 ml.
Determination of sulfur compounds by HPLC.
Sulfur compounds (sulfide, thiosulfate, sulfite and sulfate) were determined by HPLC (Thermo Separation Products) using the method of Rethmeier et al. (1997). Sulfate was measured in culture supernatants.
Determination of protein concentration.
The protein concentration of fermenter samples of Alc. vinosum was determined using Bradford reagent (Sigma). Liquid culture (1 ml) was centrifuged (15 700 g, 4 min) and the pellet was resuspended in 1 ml 1 M NaOH. The sample was incubated for 5 min at 95 °C and centrifuged again (15 700 g, 3 min). Supernatant (25 µl) was mixed with 750 µl Bradford reagent and incubated for 10 min. The absorbance was measured at 595 nm against a reagent blank. A calibration curve was recorded for the range 0–1.4 mg BSA ml–1.
(XANES) spectroscopy – experimental.
XANES spectra were recorded at the Double Crystal Monochromator beamline of the Center for Advanced Microstructures and Devices (CAMD), Baton Rouge, LA (Hormes et al., 2006). CAMD's storage ring was operated at an energy of 1.3 GeV with electron currents between 200 and 80 mA. The synchrotron radiation was monochromatized by a modified Lemonnier-type monochromator (Lemonnier et al., 1978) equipped with InSb (111) crystals. The monochromatic flux rate per second on the sample was about 5x108 photons (at 100 mA). Measurements (repeated twice) were performed in transmission mode with ionization chambers (60 mbar air pressure inside). For energy calibration, the spectrum of zinc sulfate was used as a ‘secondary standard’, setting the maximum of the first resonance (white line) to an energy of 2481.44 eV. (reproducibility ±0.1 eV). Spectra were scanned with step widths of 0.5 eV between 2440 and 2468 eV, 0.1 eV between 2468 and 2485 eV, and 0.3 eV between 2490 and 2520 eV (integration time 1 s). Using the Origin program (OriginLab), a linear background determined in the pre-edge region was subtracted from the raw data to correct the spectra for contributions from higher shells and from supporting materials. Spectra were normalized at 2510 eV.
Quantitative analysis of spectra.
The interactive fitting and plotting packages Mn-Fit 4.04/15 (available at http://www-zeus.physik.uni-bonn.de/
brock/mn_fit.html) and WINXAS (Ressler, 1998) were used. Both programs provide tools to find the minimum value of a multi-parameter function and to analyse the shape of the function around the minimum. Both packages yield comparable results (percentage contributions). A set of five reference spectra was used for fitting the bacterial spectra (reduced and oxidized glutathione, cyclo-octasulfur, polymeric sulfur and zinc sulfate), and a set of two reference spectra for fitting the elemental sulfur (cyclo-octasulfur, polymeric sulfur), respectively. A chi square criterion was applied to find the linear combination of these spectra that reproduced the XANES spectrum of interest with the highest probability. Further details concerning the quantitative analysis, especially its verification, potential and restrictions, have been published previously (Prange et al., 2002a, 2003). The errors of the percentage contributions of sulfur species (Table 1) can be estimated to be <10 % (absolute value) (Prange et al., 2002a; 2003).
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, 2002a).
Reference compounds.
Glutathione (oxidized and reduced) and zinc sulfate were used as reference compounds for fitting the spectra. These compounds were of reagent grade, purchased from Sigma and used as received. Furthermore, pure cyclo-octasulfur (S8 rings) and polymeric sulfur were used as reference compounds, both kindly provided by Professor Dr R. Steudel. The reference compounds were ground into fine powder and placed homogeneously on a Kapton film (see above).
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RESULTS AND DISCUSSION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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Firstly, the exact chemical speciation of the two forms of elemental sulfur (S1 and S2) that were used for the growth experiments was analysed by XANES spectroscopy to determine the ratios of S8 rings to polymeric sulfur. Fig. 1(a, b) shows the sulfur K-edge XANES spectra of pure cyclo-octasulfur and pure polymeric sulfur, respectively. Fig. 2 shows the sulfur K-edge XANES spectra of the two different batches of elemental sulfur and the corresponding fits. The quantitative analyses of the spectra showed that the two forms of elemental sulfur differed significantly in the ratio of S8 rings and polymeric sulfur (Table 1
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). During that time, protein concentration increased from 0.106 to 0.738 mg ml–1 (Table 3), indicating growth of the culture with elemental sulfur as the single sulfur source. Further uptake and oxidation of elemental sulfur did not occur in the following 24 h, and small sulfur platelets remained in the culture (see below). Only 37 mM sulfate was formed from 50 mM sulfur, showing that only 74 % of the added elemental sulfur was taken up and oxidized to sulfate. Taking an error of ±10 % of the quantitative analysis into account, this result fits quite well to the 68 % polymeric sulfur fraction in S1 (Table 1). The protein concentration of the culture increased only marginally after oxidation of the intracellular stored sulfur, also indicating that further sulfur oxidation did not occur in that time. The occurrence of sulfur platelets was observed about 30 h after the addition of elemental sulfur; they remained in the culture and were not taken up by Alc. vinosum. A corresponding formation of sulfur platelets was never observed in control experiments without cells. XANES analyses of these remaining sulfur platelets showed that they consisted almost completely (95 %) of S8 rings (Fig. 3a, Table 1). The level of polymeric chains in the remaining sulfur platelets was only 3 %, compared to 68 % at the beginning of the experiment.
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) showed that the polymeric chain fraction was about 39 % (Table 1) and therefore significantly lower than that of S1. Dispersion of the added elemental sulfur, formation of the sulfur globules, generation of sulfur platelets and the beginning of sulfate production were comparable to those of the first experiment. Sulfur platelets also remained in the culture and were not taken up and oxidized by Alc. vinosum. A constant amount of 22.6 mM sulfate was determined for 153 h, corresponding to 45 % of the expected amount of sulfate. The added elemental sulfur contained 39 % polymeric sulfur (compared to 45 % sulfate) and the remaining sulfur platelets mostly consisted of cyclo-octasulfur (Tables 1 and 2).
Both sets of growth experiments yielded evidence that Alc. vinosum can use only polymeric sulfur, or at least strongly prefers this sulfur species when growing on elemental sulfur. This interpretation was supported by the observation that cultures of Alc. vinosum fed with pure polymeric sulfur (50 mM) took up the added sulfur completely, formed intracellular sulfur globules, and completely oxidized the stored sulfur to 49 mM sulfate in the medium. Sulfur platelets did not appear after degradation of the sulfur globules. To make sure that the speciation in the sulfur globules of Alc. vinosum grown on elemental sulfur was comparable to that of Alc. vinosum grown on sulfide, we investigated Alc. vinosum cells (with internal sulfur globules) by XANES spectroscopy (Fig. 3c). Fitting the spectrum with five reference compounds (Fig. 1, Table 1) yielded a sulfur speciation very similar to that obtained for Alc. vinosum cells grown on sulfide (Prange et al., 2002a). Only the monosulfane : disulfane ratio differed, and this can be attributed to physiological variability (cf. Prange et al., 2002b).
Our observation that Alc. vinosum only uses, or at least strongly prefers, polymeric sulfur (sulfur chains) is in contrast to the findings of Laishley et al. (1986) for Atb. albertensis, which has the ability to oxidize cyclo-octasulfur as well as polymeric sulfur. However, it has to be kept in mind that Atb. albertensis is an aerobic, chemotrophic sulfur-oxidizing bacterium that grows at low pH, and therefore differences in elemental sulfur use can be expected. While Atb. albertensis is supposed to be more affected by the sulfur crystal microstructure than by its specific molecular composition (Laishley et al., 1986), the ability to take up elemental sulfur in the phototrophic bacterium Alc. vinosum seems to be completely dependent on the specific molecular composition of the elemental sulfur. On the basis of the results presented in this study for Alc. vinosum, one might speculate that sulfur chains are the microbiologically preferred form of elemental, zero valent sulfur for other sulfur-oxidizing bacteria also. Our hypothesis gains some support from the recent study of Urich et al. (2006). They investigated the influence of different sulfur species on enzyme functions in the sulfur oxygenase reductase from the thermoacidophilic archaeon Aquifex aeolicus. Theoretical considerations on the basis of the crystal structure of this enzyme led to the hypothesis that linear, but not cyclic, sulfur species can serve as a substrate for this enzyme. Furthermore, sulfur globules of Alc. vinosum consist exclusively of sulfur chains (mono-bis-organyl sulfanes) that are gradually shortened during oxidation of the sulfur stored in the sulfur globules (Prange et al., 2002a, b). Therefore, it appears that the organism is completely unable to deal with sulfur rings, the chemically more stable cyclo-octasulfur, outside or inside the cell. A different mechanism (instead of direct uptake of elemental sulfur) has been hypothesized for the green sulfur bacterium Prosthecochloris vibrioformis. In this bacterium, sulfide appears as an intermediate during elemental sulfur oxidation (Borkenstein & Fischer, 2006), which corresponds to the earlier findings of Paschinger et al. (1974) for Chlorobium limicola. In Alc. vinosum, however, we did not find evidence in the XANES spectra for the formation of intermediates such as sulfide or polysulfides during uptake of elemental sulfur. Furthermore, sulfide was not detectable by the very sensitive detection technique as a bimane derivative. Finally, to the best of our knowledge, no enzymes have been reported which can perform a sulfur ring cleavage or sulfur ring activation.
For some thiobacilli able to use elemental sulfur, direct cell–sulfur contact is necessary for its oxidation (Schaeffer et al., 1963; Bryant et al., 1984; Espejo & Romero, 1987). Therefore, it was investigated whether Alc. vinosum also needs direct cell–sulfur contact for elemental sulfur oxidation. The elemental sulfur (S1) was separated from the cells by a dialysis membrane with a 100 kDa cut-off. This completely prevented the formation of sulfur globules. Sulfate was not detected during 84 h, and the protein concentration did not increase, showing that the culture did not grow during that time. This indicates that it is necessary for Alc. vinosum to be attached to elemental sulfur particles, and that direct cell–sulfur contact is a prerequisite for the uptake of elemental sulfur (see Note Added in Proof). The mechanism of uptake, however, is completely unknown. Only a few studies of the elemental sulfur adhesion mechanisms of chemotrophic sulfur oxidizers are available. Takakuwa et al. (1979) have shown for Acidithiobacillus thiooxidans that thiol groups are probably involved in the process. Also, the adhesion ability seems to be energy dependent. For Atb. albertensis, Bryant et al. (1984) have shown that adhesion to elemental sulfur is due to an interaction between the glycocalyx of the organism and the sulfur surface. In other studies, Ohmura et al. (1996) and Ramírez et al. (2004) have found evidence for an outer-membrane protein in Acidithiobacillus ferrooxidans (formerly Thiobacillus ferrooxidans) which is probably involved in cell–sulfur adhesion. Extracellular polymeric substances (LPSs), which have been found in Atb. ferrooxidans and Atb. thiooxidans when attached to pyrite (Gehrke et al., 1998; Harneit et al., 2006), might have a similar function. Experiments to investigate the adhesion of elemental sulfur to phototrophic bacteria, however, have been not carried out so far, but will be performed in future in Alc. vinosum.
Conclusions
Our results show that the molecular composition of elemental sulfur is essential for its uptake and oxidation in Alc. vinosum. This organism can only use, or at least strongly prefers, the polymeric sulfur fraction (sulfur chains) when taking up elemental sulfur. Furthermore, direct cell–sulfur contact is needed for the uptake of elemental sulfur. Probably, speciation of elemental sulfur plays an important role in a more general way and we hypothesize that sulfur chains are also the microbiologically preferred form when elemental sulfur is taken up and oxidized by other micro-organisms. In our ongoing research, the utilization, mobilization and oxidation of elemental sulfur by other sulfur-oxidizing bacteria will be investigated.
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NOTE ADDED IN PROOF |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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ACKNOWLEDGEMENTS |
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Edited by: H. L. Drake
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONNOTE ADDED IN PROOFREFERENCES |
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Received 31 October 2006;
revised 18 December 2006;
accepted 22 December 2006.
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