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Genetic and physiological characterization of the Borrelia burgdorferi ORF BB0374-pfs-metK-luxS operon, by S. P. Riley, T. Bykowski, K. Babb, K. von Lackum and B. Stevenson

Microbiology vol. 153, part 7, pp. 2304 - 2311

Fig. S1. (A) Q-RT-PCR analysis of luxS transcription by wild-type (wt), rpoN^- and rpoS^- B. burgdorferi. (B) The control gene ospC was not transcribed by either mutant, consistent with previous results. All results were standardized relative to flaB mRNA levels. Error bars indicate one standard deviation from the mean. All analyses were performed a minimum of three times. Raw data used to generate the graphs are presented in Table S2 [PDF] (24 kb).

Table S1. Raw data for the Q-RT-PCR analysis of ORF BB0374, pfs, metK, luxS and flaB transcript levels from Borrelia burgdorferi strain B31-MI-16 grown for 2, 4, 6 or 8 days at 34 °C. [PDF] (31 kb).

Table S2. Raw data and calculations for Q-RT-PCR analyses of luxS, ospC and flaB transcript levels from Borrelia burgdorferi strains B31-A3 (wt), A3ntrA (rpoN) and A34rpoS (rpoS) grown at 34 °C. [PDF] (11 kb).

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