Comparative genomic analysis of mycobacteriophage Tweety: evolutionary insights and construction of compatible site-specific integration vectors for mycobacteria, by T. T. Pham, D. Jacobs-Sera, M. L. Pedulla, R. W. Hendrix and G. F. Hatfull
Microbiology vol. 153, part 8, pp. 2711 - 2723
Fig. S1. Alignment of Tweety genes 20 and 21 with Che8 gene 21. The Che8 gene 21 sequence is shown in green, and Tweety 20 and 21 in red and blue respectively. A single nucleotide deletion in Tweety (boxed) results in a frameshift to generate the truncated Tweety gp20. [PDF] (620 kb)
Fig. S2. Sequence chromatograms confirming a nucleotide deletion in the Tweety genome relative to the homologous segment in phage Llij (which is identical to Che8 in this region). [PDF] (1 kb)
Fig. 3 (larger version). Map of the Tweety genome and comparison to maps of Che8, Llij, PMC and Che9d. Genomes are represented by horizontal lines with putative genes shown as boxes above (transcribed rightwards) or below (transcribed leftwards) each genome; the number of each gene is shown within each box. The diagonal arrow indicates a programmed translational frameshift between Tweety genes 12 and 13. All genes have been assorted into phamilies (Phams) of related sequences using the computer program 'Phamerator' (S. Cresawn, R. W. Hendrix & G. F. Hatfull, unpublished data); the phamily number is displayed above each gene and the boxes colour-coordinated accordingly. Note that the Pham numbers differ from those described previously (Hatfull et al., 2006). Putative gene functions are noted. [PDF] (2771 kb)
Table S1. Nucleotide repeats in Tweety gene 54 [PDF] (12 kb)
Table S2. Repeated sequences in phages Tweety gp54, Che8 gp57 and PMC gp51 [PDF] (13 kb)
Copyright © 2009 Society for General Microbiology.
