A new promoterless reporter vector reveals antisense transcription in Mycoplasma genitalium, by M. Lluch-Senar, M. Vallmitjana, E. Querol and J. Piñol
Microbiology vol. 153, part 8, pp. 2743 - 2752
Table S1. Transposon insertions found inside M. genitalium coding regions [PDF] (12 kb)
Fig. S1. Transposon insertion sites found in the M. genitalium genome. The red downward-facing triangles in the upper line indicate transposon insertion sites in the plus strand; the blue upward-facing triangles indicate transposon insertion sites insertion in the minus strand. The transcription terminator sequences were determined by the TransTerm program (Ermolaeva et al., 2000) and are marked by a T facing up (terminator sequence in the plus strand) or a T facing down (terminator sequence in the minus strand). A "T" symbol in red indicates the terminator sequence determined experimentally by Benders et al. (2005). MgPa islands I to VIII contain sequences derived from mg191 and mg192 ORFs. The MgPa191 island exhibits only a short stretch of a sequence related to the mg191 ORF. [PDF] (2245 kb)
Fig. S2. BLAST alignment of sequences in MgPa island VI surrounding the transcriptional start point and mg192 gene in the MgPa operon. The sequences in the alignment are from the minus strand of the M. genitalium genome and are numbered starting at base 314,465 (MgPa island VI) or 226,395 (mg192) of the M. genitalium complete genome. The heterogeneous transcriptional start point is boxed in green and the -10 region of a putative promoter, in yellow. [PDF] (572 kb)
REFERENCES
Benders, G. A., Powell, B. C. & Hutchison, C. A., 3rd (2005). Transcriptional analysis of the conserved ftsZ gene cluster in Mycoplasma genitalium and Mycoplasma pneumoniae. J Bacteriol 187, 4542-4551.
Ermolaeva, M. D., Khalak, H. G., White, O., Smith, H. O. & Salzberg, S. L. (2000). Prediction of transcription terminators in bacterial genomes. J Mol Biol 301, 27-33.
Copyright © 2009 Society for General Microbiology.
