| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Mini-Review |
1 Department of Biology (Area 10), University of York, PO Box 373, York YO10 5YW, UK
2 Molecular Infectious Diseases Group, University of Oxford Department of Paediatrics, Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DS, UK
Correspondence
Gavin H. Thomas
ght2{at}york.ac.uk
 |
ABSTRACT |
|---|
 TOP ABSTRACT IntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
|
Introduction |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
), although there are numerous naturally occurring variations including substitutions at carbon 5, or covalent modifications of the sugar's hydroxyls (for a more general overview of the diversity of sialic acid forms in nature see Angata & Varki, 2002). Most experimental work on bacterial sialic acid metabolism relates to Neu5Ac, therefore the term ‘sialic acid’ has been used in the literature to mean this particular chemical primarily. For the sake of simplicity we will not attempt to distinguish between these two terms; however, bacteria may encounter other sialic acid forms in their environment and process them through the same pathways as used for Neu5Ac metabolism. This review will summarize recent literature describing how pathogenic bacteria use sialic acid in their lifestyles and will draw primarily from a limited number of well-studied pathogens, including Escherichia coli K1, Haemophilus influenzae, Pasteurella multocida, Neisseria spp., Campylobacter jejuni and Streptococcus agalactiae. These bacteria use sialic acid for a variety of different purposes that play important roles in their ability to colonize, persist and cause disease in mammalian species.
|
)]. The mini-review will consider recent discoveries in three areas related to pathogenic bacteria and sialic acid, namely (i) how bacteria acquire sialic acid, either by scavenging from the environment or by de novo biosynthesis, (ii) how bacteria incorporate this compound into their cell-surface features, and (iii) how these sialic acid-bearing surfaces modify the interaction of the bacterium with its host. |
Preparing the supply line – sialic acid acquisition and biosynthesis |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
) (Vimr & Lichtensteiger, 2002; Vimr et al., 2004). The first route is used by a number of bacteria including E. coli K1, Neisseria meningitidis and C. jejuni (Vimr et al., 2004). The metabolite UDP-GlcNAc is the precursor for sialic acid biosynthesis (Fig. 1; abbreviations for the names of metabolites are reported in the legend) and is produced by most cells, as it is used in cell wall biosynthesis; the combined actions of the NeuC and NeuB proteins convert this to Neu5Ac via ManNAc (Fig. 1).
The second source of sialic acid is the environment, which for the pathogens mentioned in this review is the mammalian host. Many pathogens secrete a sialidase that releases sialic acid from a diverse range of host sialoglycoconjugates (Fig. 1) (Corfield, 1992); however, other sialic acid-utilizing bacteria, such as the respiratory pathogen H. influenzae, lack genes for a sialidase yet are reliant on host-derived sialic acid (Bouchet et al., 2003). Presumably free sialic acid is made available to such pathogens by other, sialidase-expressing bacteria living in the same niche (Shakhnovich et al., 2002), or, as hypothesized by Sohanpal et al. (2004, 2007), by host sialidases that are activated in the course of inflammation. The latter process is part of the normal recycling of sialic acid and there has been a recent suggestion that the host cells might use free sialic acid to help them cope with oxidative stress (Iijima et al., 2007).
Regardless of the mode of release of free monomeric sialic acid, bacteria must then capture this resource using specific transporters (Fig. 1). The one exception to this is Neisseria gonorrhoeae, which appears to have obviated the need for uptake by secreting the enzymes that sialylyate its LPS and by using the activated form of sialic acid, CMP-Neu5Ac, from the host as a substrate (see next section). Our understanding of sialic acid transport in bacteria has until recently been quite poor and, at the molecular level, limited to the characterization of the NanT sialic acid transporter from E. coli K-12 (Vimr et al., 2004). This is a classical secondary transporter of the major facilitator superfamily (MFS) and is essential for Neu5Ac uptake by E. coli (Fig. 1) (Vimr et al., 2004). The route of sialic acid uptake in H. influenzae and P. multocida has recently been established; it is dependent on a tripartite ATP-independent periplasmic (TRAP) transporter, SiaPQM (Steenbergen et al., 2005; Allen et al., 2005; Severi et al., 2005). This family of transporters are unusual as they are secondary transporters like NanT, but utilize an extracytoplasmic solute receptor (ESR) protein in the transport mechanism (Fig. 1). The ESR component of the H. influenzae sialic acid transporter, SiaP, binds sialic acid with high affinity and specificity (Severi et al., 2005), and the structure of this protein revealed a fold not dissimilar to that seen in other ESR proteins from ATP-binding cassette (ABC) transporters (Muller et al., 2006). These data suggest that SiaPQM functions as a high-affinity transporter in vivo, which would be consistent with the scavenging requirements of H. influenzae. The sialic acid transporter from Haemophilus ducreyi (SatABCD) has also been characterized; interestingly, it is not a TRAP transporter but rather is an ABC transporter and hence presumably of high affinity (Fig. 1) (Post et al., 2005). Therefore it appears that bacteria have evolved multiple routes to capture sialic acid from the environment, supporting the importance of this process in vivo. This is certainly true for organisms such as H. influenzae and H. ducreyi, which are unable to synthesize sialic acid, and rely on their respective transporters for the provision of sialic acid to the LPS sialylation pathways (Severi et al., 2005; Allen et al., 2005; Post et al., 2005). As we explain below, LPS sialylation is an important virulence factor for some pathogens, which again stresses the importance of sialic acid uptake in the lifestyle of these bacteria.
There has also been a recent characterization of the Neu5Ac-inducible porin NanC (YjhA) from E. coli K-12, which is essential for growth on Neu5Ac when both the general OmpC and OmpF porins are not expressed (Fig. 1) (Condemine et al., 2005). However, the occurrence of this porin is not widespread and the role of Neu5Ac-specific transport across the outer membrane of bacterial pathogens has not been established.
In addition to ‘preparing the supply line’ for making sialic acid-modified cell surfaces, some bacteria, such as H. influenzae and E. coli, can also utilize the transported sialic acid as a carbon and nitrogen source (Vimr et al., 2004; Severi et al., 2005). The pathway by which this occurs has been well characterized and uses the N-acetylneuraminate aldolase NanA that cleaves Neu5Ac to ManNAc and pyruvate (Fig. 1). ManNAc is then ultimately converted to fructose 6-phosphate and ammonia, via the action of the NanK, NanE, NagB and NagA proteins, where it can enter central metabolism (Fig. 1) (Vimr et al., 2004). How these bacteria maintain the balance between the catabolic and anabolic pathways is not clearly defined, nor is it clear how sialic acid catabolism contributes to virulence. There is evidence that in H. influenzae sialic acid catabolism competes with the LPS sialylation pathway for sialic acid that has been transported into the cell (Fig. 1), as demonstrated by the LPS hyper-sialylation phenotype and increased fitness in an animal model of a nanA mutant (Vimr et al., 2000). In E. coli K1 catabolism has the potential to compete with the polysialic acid (PSA) synthesis pathway (Fig. 1); however, whereas the neu genes are expressed constitutively the nan genes are induced by Neu5Ac (Vimr et al., 2004), and the hypothesis of tight coupling of Neu5Ac synthesis and activation by NeuB/NeuA might explain the lack of a futile cycle of Neu5Ac synthesis and degradation, and implies that the catabolic operon is expressed only when exogenous sialic acid is made available (Vimr et al., 2004). Interestingly Chang et al. (2004) have shown that the nanAT genes are required for E. coli colonization of the mouse colon, where sialic acid-rich mucin appears to be an important nutrient source in vivo. However, studies on the contribution of sialic acid catabolism to virulence using animal models must be interpreted with caution as the distribution and nature of sialic acid may be significantly different in the human host, which affects any possible extrapolation.
|
Deploying sialic acid onto the battlefield – synthesis of sialic acid-containing cell-surface features |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
). As mentioned above, N. gonorrhoeae relies instead on an outer-membrane-associated sialyltransferase to scavenge CMP-Neu5Ac directly from the secretions of the host (Fig. 1) (Shell et al., 2002).
A significant part of our knowledge about the incorporation of sialic acid into bacterial macromolecules has come from the analyses of the processes for synthesis of the PSA capsules of E. coli and N. meningitidis. In E. coli, NeuA activates Neu5Ac prior to its incorporation into the K1 and K92 capsules while the N. meningitidis orthologue carries out the analogous function relating to both capsule and LPS synthesis (Fig. 1). In E. coli, NeuS functions as the main polysialyltransferase adding Neu5Ac to oligosialic acid receptors to form the PSA capsule, which is then exported through the Kps system (Fig. 1) (Vimr et al., 2004). An initial step in PSA synthesis is thought to include the addition of Neu5Ac by the NeuE protein to some as-yet-unidentified initiator molecule, presumed to be a lipid (Vimr et al., 2004). Once synthesized, sialic acid residues in the PSA capsule of both N. meningitidis and E. coli can be modified by O-acetylation (Claus et al., 2004; Steenbergen et al., 2006; Deszo et al., 2005), an emerging and exciting new feature of sialic acid metabolism also seen in unencapsulated organisms (see below). In E. coli the O-acetyltransferases NeuO and NeuD modify PSA and monomeric Neu5Ac, respectively, the latter of which can be deacetylated by NeuA acting as a bifunctional enzyme (Fig. 1) (Steenbergen et al., 2006; Deszo et al., 2005; Bergfeld et al., 2007). These new discoveries highlight the huge potential diversity of capsule structures that may be presented on the cell surface by varying the patterns of acetylated sialic acid (King et al., 2007). The only Gram-positive bacterium reported to produce a sialic acid-containing capsule is S. agalactiae, which possesses a sialyltransferase (CpsK) that adds a terminal 
-2,3-linked Neu5Ac to galactose within the capsule's oligosaccharide repeat (Chaffin et al., 2005). Again, recent work has shown that this Neu5Ac residue can be modified by O-acetylation (Lewis et al., 2006).
Sialylation of the LPS is also catalysed by linkage-specific sialyltransferases. Both N. meningitidis and N. gonorrhoeae sialylate their LPS through the outer-membrane -2,3 sialyltransferase Lst (Fig. 1), although the enzyme is expressed at a higher level in the gonococcus (Packiam et al., 2006). LPS sialylation is also a feature of several pathogenic members of the Pasteurellaceae, including H. ducreyi, H. influenzae, Haemophilus somnus and P. multocida. H. influenzae has the greatest number of characterized LPS sialyltransferases, with up to four being present in any given strain (Fox et al., 2006). The main sialyltransferase is Lic3A (Fig. 1), which adds -2,3-Neu5Ac (Hood et al., 2001), and has been shown to be absolutely required for bacterial survival in the middle ear in an animal model of otitis media (Bouchet et al., 2003). Amongst the other sialyltransferases, the Lic3A-homologue Lic3B is a bifunctional enzyme that can add mono- or disialic acid to the LPS acceptor (Fig. 1) (Fox et al., 2006). C. jejuni also possesses mono- or bifunctional LPS sialyltransferases transferring either -2,3-Neu5Ac or disialic acid (Gilbert et al., 2000). It is of note that the terminal sialic acid residue in the disialylated LPS can also be modified by an O-acetyltransferase (Fig. 1) (Houliston et al., 2006). Sialic acid, also in its O-acetylated form, can be included as part of the oligosaccharides that are repeated to form the O-antigen of LPS in E. coli (Ali et al., 2006), although the enzymes required for this process are not well studied.
While sialylated LPS and PSA capsules have obvious benefits to bacteria in particular host compartments, for example in evading the immune response as we describe below, their presence may not always be desirable when the organism is within other compartments either inside or outside the host. Some pathogens, such as Neisseria spp., H. influenzae and C. jejuni, regulate expression of cell-surface features by stochastic genetic mechanisms relying on rapid and reversible ON to OFF switching of the expression of key biosynthetic genes, the so-called ‘phase variation’ (van der Woude & Baumler, 2004). Phase variation also modulates O-acetylation of the PSA capsule of E. coli K1 (Deszo et al., 2005; Bergfeld et al., 2007). Such variable expression makes it likely that any population of bacteria will be expressing alternative glycoforms and that any one expression pattern might confer a fitness advantage to the relevant bacteria under any given conditions.
|
Contact with the enemy – alteration of host responses by sialic acid-coated bacteria |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
).
The PSA capsule of N. meningitidis serogroup B and E. coli K1 is poorly immunogenic and this is thought to be a consequence of its being structurally identical to the PSA chains of the mammalian neuronal cell adhesion molecule, NCAM (Vimr & Lichtensteiger, 2002). The exact mechanisms by which the PSA capsule helps evade the host immune response are not clear, but in meningococci this capsule is required for resistance against the killing effect of human serum, possibly by hindering the insertion of the complement membrane-attack complex (MAC) in the bacterial membrane (Vimr & Lichtensteiger, 2002). The PSA capsule of E. coli K1 does not confer serum resistance, but it inhibits opsonization and phagocytosis in vivo (Fig. 1) (Vimr & Lichtensteiger, 2002). The sialylated capsule of S. agalactiae has similar inhibitory effects on phagocytosis and acts by impairing C3 deposition on the cell surface, thus preventing activation of the complement alternative pathway (Marques et al., 1992).
LPS sialylation also inhibits the complement alternative pathway in both N. gonorrhoeae and in non-typable H. influenzae (NTHi), although these two pathogens use different mechanisms to this effect (Figueira et al., 2007; Ram et al., 1998). Gonococcal sialylated LPS increases the binding to the bacterial cell surface of factor H (fH), an anti-activator of the complement alternative pathway (Fig. 1) (Ram et al., 1998). This strategy mimics the effect that sialylation has on some eukaryotic cell membranes, which self-protect from C3 attack by recruiting fH, and results in higher serum resistance (Ram et al., 1998). To date it is not known whether the sialylated LPS is an actual binding site for fH (Fig. 1) (Madico et al., 2007). Interestingly, the positive effect of LPS sialylation on fH binding requires the presence of the gonococcal porin PorB (Fig. 1), suggesting that sialylated LPS and PorB might constitute a composite epitope, or that sialylation might cause a conformational change in the LPS that unmasks novel sites in PorB (Madico et al., 2007). In NTHi, LPS sialylation inhibits deposition of C3 without entailing fH binding (Fig. 1) (Figueira et al., 2007). Restoration of virulence to an avirulent, sialic acid-free mutant of NTHi inoculated into complement-depleted chinchillas demonstrated a role for the complement in eliminating unsialylated H. influenzae in vivo (Figueira et al., 2007). Circumstantial evidence suggests that one target of C3 on unsialylated cell surfaces might be LPS, raising the possibility that sialylation acts by masking those sites (Figueira et al., 2007).
There is evidence that the sialylated bacterial surfaces of C. jejuni, N. meningitidis and S. agalactiae (LPS for the former two species and capsule for the latter) can interact with sialic acid-specific lectins of the Siglec family, which are normally expressed on the surface of cell types of the immune system (Avril et al., 2006; Carlin et al., 2007; Jones et al., 2003). This has led to the hypothesis of direct cellular interactions between these cell types and the bacterial cells, which may represent yet another way by which bacterial cell-surface sialylation might modulate the host's immune response (Avril et al., 2006; Carlin et al., 2007; Jones et al., 2003).
|
Concluding remarks |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
, 2004) has demonstrated a different way by which sialic acid may modify bacterial cell surfaces. In uropathogenic strains of E. coli, free Neu5Ac inhibits the phase-variable expression of type 1 fimbriae, which are known virulence factors mediating adherence to and invasion into epithelial cells (Sohanpal et al., 2004). This is achieved by antagonizing the activator effect of key N-acetylsugar-responsive transcription factors (NanR and NagC) on the fimB gene, which is in turn a regulator of the expression of the fim operon (Sohanpal et al., 2004). As sialic acid is produced by the host in the course of inflammation, it is possible that a sialidase-negative bacterium such as E. coli recognizes free sialic acid as an indicator of inflammation, and downregulates expression of adhesins in response (Sohanpal et al., 2004, 2007). These recent discoveries along with new work on sialic acid transport, sialic acid acetylation and the mechanisms of serum resistance continue to further our understanding of sialic acid in biology and specifically of how it is exploited by pathogenic bacteria for a variety of different purposes in vivo.
|
ACKNOWLEDGEMENTS |
|---|
|
REFERENCES |
|---|
|
TOPABSTRACTIntroductionPreparing the supply line...Deploying sialic acid onto...Contact with the enemy...Concluding remarksREFERENCES |
|---|
Allen, S., Zaleski, A., Johnston, J. W., Gibson, B. W. & Apicella, M. A. (2005). Novel sialic acid transporter of Haemophilus influenzae. Infect Immun 73, 5291–5300.
Angata, T. & Varki, A. (2002). Chemical diversity in the sialic acids and related alpha-keto acids: an evolutionary perspective. Chem Rev 102, 439–469.[CrossRef][Medline]
Avril, T., Wagner, E. R., Willison, H. J. & Crocker, P. R. (2006). Sialic acid-binding immunoglobulin-like lectin 7 mediates selective recognition of sialylated glycans expressed on Campylobacter jejuni lipooligosaccharides. Infect Immun 74, 4133–4141.
Bergfeld, A. K., Claus, H., Vogel, U. & Muhlenhoff, M. (2007). Biochemical characterization of the polysialic acid specific O-acetyltransferase NeuO of Escherichia coli K1. J Biol Chem
Bouchet, V., Hood, D. W., Li, J., Brisson, J. R., Randle, G. A., Martin, A., Li, Z., Goldstein, R., Schweda, E. K. & other authors (2003). Host-derived sialic acid is incorporated into Haemophilus influenzae lipopolysaccharide and is a major virulence factor in experimental otitis media. Proc Natl Acad Sci U S A 100, 8898–8903.
Carlin, A. F., Lewis, A. L., Varki, A. & Nizet, V. (2007). Group B streptococcal capsular sialic acids interact with siglecs (immunoglobulin-like lectins) on human leukocytes. J Bacteriol 189, 1231–1237.
Chaffin, D. O., Mentele, L. M. & Rubens, C. E. (2005). Sialylation of group B streptococcal capsular polysaccharide is mediated by cpsK and is required for optimal capsule polymerization and expression. J Bacteriol 187, 4615–4626.
Chang, D. E., Smalley, D. J., Tucker, D. L., Leatham, M. P., Norris, W. E., Stevenson, S. J., Anderson, A. B., Grissom, J. E., Laux, D. C. & other authors (2004). Carbon nutrition of Escherichia coli in the mouse intestine. Proc Natl Acad Sci U S A 101, 7427–7432.
Claus, H., Borrow, R., Achtman, M., Morelli, G., Kantelberg, C., Longworth, E., Frosch, M. & Vogel, U. (2004). Genetics of capsule O-acetylation in serogroup C, W-135 and Y meningococci. Mol Microbiol 51, 227–239.[CrossRef][Medline]
Condemine, G., Berrier, C., Plumbridge, J. & Ghazi, A. (2005). Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli. J Bacteriol 187, 1959–1965.
Corfield, T. (1992). Bacterial sialidases – roles in pathogenicity and nutrition. Glycobiology 2, 509–521.
Deszo, E. L., Steenbergen, S. M., Freedberg, D. I. & Vimr, E. R. (2005). Escherichia coli K1 polysialic acid O-acetyltransferase gene, neuO, and the mechanism of capsule form variation involving a mobile contingency locus. Proc Natl Acad Sci U S A 102, 5564–5569.
Figueira, M. A., Ram, S., Goldstein, R., Hood, D. W., Moxon, E. R. & Pelton, S. I. (2007). Role of complement in defense of the middle ear revealed by restoring the virulence of nontypeable Haemophilus influenzae siaB mutants. Infect Immun 75, 325–333.
Fox, K. L., Cox, A. D., Gilbert, M., Wakarchuk, W. W., Li, J., Makepeace, K., Richards, J. C., Moxon, E. R. & Hood, D. W. (2006). Identification of a bifunctional lipopolysaccharide sialyltransferase in Haemophilus influenzae: incorporation of disialic acid. J Biol Chem 281, 40024–40032.
Gilbert, M., Brisson, J. R., Karwaski, M. F., Michniewicz, J., Cunningham, A. M., Wu, Y., Young, N. M. & Wakarchuk, W. W. (2000). Biosynthesis of ganglioside mimics in Campylobacter jejuni OH4384. Identification of the glycosyltransferase genes, enzymatic synthesis of model compounds, and characterization of nanomole amounts by 600-MHz 1H and 13C NMR analysis. J Biol Chem 275, 3896–3906.
Harvey, H. A., Swords, W. E. & Apicella, M. A. (2001). The mimicry of human glycolipids and glycosphingolipids by the lipooligosaccharides of pathogenic Neisseria and Haemophilus. J Autoimmun 16, 257–262.[CrossRef][Medline]
Hood, D. W., Cox, A. D., Gilbert, M., Makepeace, K., Walsh, S., Deadman, M. E., Cody, A., Martin, A., Mansson, M. & other authors (2001). Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae. Mol Microbiol 39, 341–350.[CrossRef][Medline]
Houliston, R. S., Endtz, H. P., Yuki, N., Li, J., Jarrell, H. C., Koga, M., van Belkum, A., Karwaski, M. F., Wakarchuk, W. W. & Gilbert, M. (2006). Identification of a sialate O-acetyltransferase from Campylobacter jejuni: demonstration of direct transfer to the C-9 position of terminal alpha-2,8-linked sialic acid. J Biol Chem 281, 11480–11486.
Iijima, R., Takahashi, H., Ikegami, S. & Yamazaki, M. (2007). Characterization of the reaction between sialic acid (N-acetylneuraminic acid) and hydrogen peroxide. Biol Pharm Bull 30, 580–582.[CrossRef][Medline]
Jones, C., Virji, M. & Crocker, P. R. (2003). Recognition of sialylated meningococcal lipopolysaccharide by siglecs expressed on myeloid cells leads to enhanced bacterial uptake. Mol Microbiol 49, 1213–1225.[CrossRef][Medline]
King, M. R., Steenbergen, S. M. & Vimr, E. R. (2007). Going for baroque at the Escherichia coli K1 cell surface. Trends Microbiol 15, 196–202.[CrossRef][Medline]
Lewis, A. L., Hensler, M. E., Varki, A. & Nizet, V. (2006). The group B streptococcal sialic acid O-acetyltransferase is encoded by neuD, a conserved component of bacterial sialic acid biosynthetic gene clusters. J Biol Chem 281, 11186–11192.
Madico, G., Ngampasutadol, J., Gulati, S., Vogel, U., Rice, P. A. & Ram, S. (2007). Factor H binding and function in sialylated pathogenic neisseriae is influenced by gonococcal, but not meningococcal, porin. J Immunol 178, 4489–4497.
Marques, M. B., Kasper, D. L., Pangburn, M. K. & Wessels, M. R. (1992). Prevention of C3 deposition by capsular polysaccharide is a virulence mechanism of type III group B streptococci. Infect Immun 60, 3986–3993.
Muller, A., Severi, E., Mulligan, C., Watts, A. G., Kelly, D. J., Wilson, K. S., Wilkinson, A. J. & Thomas, G. H. (2006). Conservation of structure and mechanism in primary and secondary transporters exemplified by SiaP, a sialic acid binding virulence factor from Haemophilus influenzae. J Biol Chem 281, 22212–22222.
Packiam, M., Shell, D. M., Liu, S. V., Liu, Y. B., McGee, D. J., Srivastava, R., Seal, S. & Rest, R. F. (2006). Differential expression and transcriptional analysis of the alpha-2,3-sialyltransferase gene in pathogenic Neisseria spp. Infect Immun 74, 2637–2650.
Post, D. M., Mungur, R., Gibson, B. W. & Munson, R. S., Jr (2005). Identification of a novel sialic acid transporter in Haemophilus ducreyi. Infect Immun 73, 6727–6735.
Ram, S., Sharma, A. K., Simpson, S. D., Gulati, S., McQuillen, D. P., Pangburn, M. K. & Rice, P. A. (1998). A novel sialic acid binding site on factor H mediates serum resistance of sialylated Neisseria gonorrhoeae. J Exp Med 187, 743–752.
Severi, E., Randle, G., Kivlin, P., Whitfield, K., Young, R., Moxon, R., Kelly, D., Hood, D. & Thomas, G. H. (2005). Sialic acid transport in Haemophilus influenzae is essential for lipopolysaccharide sialylation and serum resistance and is dependent on a novel tripartite ATP-independent periplasmic transporter. Mol Microbiol 58, 1173–1185.[CrossRef][Medline]
Shakhnovich, E. A., King, S. J. & Weiser, J. N. (2002). Neuraminidase expressed by Streptococcus pneumoniae desialylates the lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: a paradigm for interbacterial competition among pathogens of the human respiratory tract. Infect Immun 70, 7161–7164.
Shell, D. M., Chiles, L., Judd, R. C., Seal, S. & Rest, R. F. (2002). The Neisseria lipooligosaccharide-specific alpha-2,3-sialyltransferase is a surface-exposed outer membrane protein. Infect Immun 70, 3744–3751.
Sohanpal, B. K., El Labany, S., Lahooti, M., Plumbridge, J. A. & Blomfield, I. C. (2004). Integrated regulatory responses of fimB to N-acetylneuraminic (sialic) acid and GlcNAc in Escherichia coli K-12. Proc Natl Acad Sci U S A 101, 16322–16327.
Sohanpal, B. K., Friar, S., Roobol, J., Plumbridge, J. A. & Blomfield, I. C. (2007). Multiple co-regulatory elements and IHF are necessary for the control of fimB expression in response to sialic acid and N-acetylglucosamine in Escherichia coli K-12. Mol Microbiol 63, 1223–1236.[CrossRef][Medline]
Steenbergen, S. M., Lichtensteiger, C. A., Caughlan, R., Garfinkle, J., Fuller, T. E. & Vimr, E. R. (2005). Sialic acid metabolism and systemic pasteurellosis. Infect Immun 73, 1284–1294.
Steenbergen, S. M., Lee, Y. C., Vann, W. F., Vionnet, J., Wright, L. F. & Vimr, E. R. (2006). Separate pathways for O-acetylation of polymeric and monomeric sialic acids and identification of sialyl O-acetyl esterase in Escherichia coli K1. J Bacteriol 188, 6195–6206.
van der Woude, M. W. & Baumler, A. J. (2004). Phase and antigenic variation in bacteria. Clin Microbiol Rev 17, 581–611.
Vimr, E. & Lichtensteiger, C. (2002). To sialylate, or not to sialylate: that is the question. Trends Microbiol 10, 254–257.[CrossRef][Medline]
Vimr, E., Lichtensteiger, C. & Steenbergen, S. (2000). Sialic acid metabolism's dual function in Haemophilus influenzae. Mol Microbiol 36, 1113–1123.[CrossRef][Medline]
Vimr, E. R., Kalivoda, K. A., Deszo, E. L. & Steenbergen, S. M. (2004). Diversity of microbial sialic acid metabolism. Microbiol Mol Biol Rev 68, 132–153.
This article has been cited by other articles:
|
|
 |
|
  S. Almagro-Moreno and E. F. Boyd Sialic Acid Catabolism Confers a Competitive Advantage to Pathogenic Vibrio cholerae in the Mouse Intestine Infect. Immun., September 1, 2009; 77(9): 3807 - 3816. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. G. Jeong, M. H. Oh, B. S. Kim, M. Y. Lee, H. J. Han, and S. H. Choi The Capability of Catabolic Utilization of N-Acetylneuraminic Acid, a Sialic Acid, Is Essential for Vibrio vulnificus Pathogenesis Infect. Immun., August 1, 2009; 77(8): 3209 - 3217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C Schoenhofen, E. Vinogradov, D. M Whitfield, J.-R. Brisson, and S. M Logan The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors Glycobiology, July 1, 2009; 19(7): 715 - 725. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Thompson, K. A. Homer, S. Rao, V. Booth, and A. H. F. Hosie An Orthologue of Bacteroides fragilis NanH Is the Principal Sialidase in Tannerella forsythia J. Bacteriol., June 1, 2009; 191(11): 3623 - 3628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. F. Carlin, S. Uchiyama, Y.-C. Chang, A. L. Lewis, V. Nizet, and A. Varki Molecular mimicry of host sialylated glycans allows a bacterial pathogen to engage neutrophil Siglec-9 and dampen the innate immune response Blood, April 2, 2009; 113(14): 3333 - 3336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mulligan, E. R. Geertsma, E. Severi, D. J. Kelly, B. Poolman, and G. H. Thomas The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter PNAS, February 10, 2009; 106(6): 1778 - 1783. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Vimr and S. M. Steenbergen Early molecular-recognition events in the synthesis and export of group 2 capsular polysaccharides Microbiology, January 1, 2009; 155(1): 9 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Choudhry, M. Bajaj-Elliott, and V. McDonald The Terminal Sialic Acid of Glycoconjugates on the Surface of Intestinal Epithelial Cells Activates Excystation of Cryptosporidium parvum Infect. Immun., August 1, 2008; 76(8): 3735 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Severi, A. Muller, J. R. Potts, A. Leech, D. Williamson, K. S. Wilson, and G. H. Thomas Sialic Acid Mutarotation Is Catalyzed by the Escherichia coli {beta}-Propeller Protein YjhT J. Biol. Chem., February 22, 2008; 283(8): 4841 - 4849. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
