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Supplementary Figure

Intoxication of epithelial cells by plasmid-encoded toxin requires clathrin-mediated endocytosis, by F. Navarro-García, A. Canizalez-Roman, J. E . Vidal and Ma. I. Salazar

Microbiology vol. 153, part 9, pp. 2828-2838

Fig. S1. Inhibition of CT and transferrin internalization by filipin or cadaverine. HEp-2 cells were preincubated for 30 min with drugs that inhibit the endocytic process. After the incubation, cells were treated with CT (0.5 μg ml-1) or transferrin (37 μg ml-1) for 3 h. The cells were fixed, permeabilized, and stained with rhodamine-phalloidin (red) and anti-CT or anti-transferrin antibodies, followed by a secondary fluorescein-labelled goat anti-rabbit antibody (green). Slides were observed by using confocal microscopy. Surface (a) and vertical (b) sections of filipin-preincubated cells treated with CT. Surface (c) and vertical (d) sections of cadaverine-preincubated cells treated with transferrin.


Fig. S1





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