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Intracellular type III secretion by cytoplasmic Shigella flexneri promotes caspase-1-dependent macrophage cell death, by G. N. Schroeder, N. J. Jann and H. Hilbi

Microbiology vol. 153, part 9, pp. 2862 - 2876

Fig. S1. Reversible inhibition by CCCP of S. flexneri growth in TSB broth. Growth of wild-type S. flexneri in TSB medium was monitored in the absence (circles) or presence (squares, triangles) of 25 μM CCCP. After 4.5 h, all cultures were washed, and CCCP was removed (squares) or added back (triangles). Growth of S. flexneri in broth was abolished by the addition of 25 μM CCCP. The inhibition was completely reversible, as removal of CCCP after 2.5 h restored growth of the bacteria at the initial rate, demonstrating that exposure to CCCP within this time frame does not kill the bacteria. The growth curve shown is representative of three independent experiments. [PDF] (72 kb)







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