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Optical mapping and 454 sequencing of Escherichia coli O157:H7 isolates linked to the US 2006 spinach-associated outbreak, by M. L. Kotewicz, M. K. Mammel, J. E. LeClerc and T. A. Cebula

Microbiology vol. 154, part 11, pp. 3518 - 3528

Fig. S1. Chromosomal markers 1 and 2 in the 2006 US spinach-associated outbreak strain, insertion sequence RFLP and RFLP in prophage H/I. Aligned fragments are highlighted in green; white fragments indicate non-alignment. [PDF] (269 kb)

Fig. S2. Chromosomal markers 3 and 9 in the 2006 US spinach-associated outbreak strain, a P4-like prophage and R prophage. Aligned fragments are highlighted in green; white fragments indicate non-alignment. As the R prophage is found within an inversion in EDL933, the EDL933 segment was inverted to show the alignment; note the reverse in the Mbp coordinates for EDL933. [PDF] (288 kb)

Fig. S3. Chromosomal markers 13 and 14 in the 2006 US spinach-associated outbreak strain, a non-insertion of a Mu-like prophage found in Sakai and an 11 kb phage scar. Sequence data demonstrates the 11 kb insertion occurred relative to the BamHI EDL933 restriction fragment at 5.150 Mbp. Aligned fragments are highlighted in green; white fragments indicate non-alignment. [PDF] (265 kb)

Fig. S4. Chromosomal markers in clinical variants of 2006 US spinach-associated outbreak, isolate EC4076, containing deletion of prophage T, and isolate EC4115, containing a 41 kb insertion within the V-like prophage at chromosome locus yehV. Aligned fragments are highlighted in green; white fragments indicate non-alignment. [PDF] (387 kb)

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