HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef

The heterotrimeric Gα protein Pga1 regulates biosynthesis of penicillin, chrysogenin and roquefortine in Penicillium chrysogenum, by R. O. García-Rico, F. Fierro, E. Mauriz, A. Gómez, M. Á. Fernández Bodega and J. F. Martín

Microbiology vol. 154, part 11, pp. 3567 - 3578

Table S1. Quantitative densitometric analysis of the Northern blot hybridization signals of the pcbC and penDE genes in Fig. 1(b) of the main paper [PDF] (57 kb)

Table S2. Quantitative densitometric analysis of the Northern blot hybridization signals of the pcbC and penDE genes in Fig. 3 of the main paper [PDF] (58 kb)

Fig. S1. Effect of modifications of pga1 on expression of the penicillin biosynthesis gene pcbAB in fermenter cultures of P. chrysogenum under controlled pH conditions. Lanes: 1, G203R-T; 2, Wis 54-1255 parental strain; 3, PgaG42R-1; 4, Δpga1. Hybridizations were performed with a probe internal to pcbAB. Note the increased expression of the pcbAB gene in the Wis 54-1255 pga1G42R transformant (lanes 3), whereas the deletion mutant Δpga1 (lanes 4) and the pga1G203R transformant (lanes 1) showed reduced expression. The rRNAs are shown in the lower part of the figure as a control of the amount of loaded RNA. [PDF] (676 kb)

Fig. S2. Penicillin production of strain Wis54-1255 growing in shake flask cultures with non-supplemented (Control) and 10 mM theophylline-supplemented (Theo-1, 2 and 3) CP medium. Error bars correspond to standard deviation of three replicates in three independent experiments. [PDF] (316 kb)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef