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Carotenoid 3′,4′-desaturase is involved in carotenoid biosynthesis in the radioresistant bacterium Deinococcus radiodurans, by B. Tian, Z. Sun, Z. Xu, S. Shen, H. Wang and Y. Hua

Microbiology vol. 154, part 12, pp. 3697 - 3706

Supplementary Fig. S1. Construction of plasmid pRADK (Gao et al., 2005).

Construction of plasmid pRADK

Supplementary Fig. S2. Scheme of construction of the mutant and confirmation of segregated mutants.

Supplementary Figs S3 and S4. Absorbance spectra of peak 2 (the major product from the wild-type) and peak 2′ (the major product from mutant R1ΔcrtD).

[PDF file of Supplementary Figs S2-S4] (225 KB)

Reference

Gao, G., Lu, H., Huang, L. & Hua, Y. (2005). Construction of DNA damage response gene pprI function deficient and function complementary mutants in Deinococcus radiodurans. Chin Sci Bull 50, 311–316.







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