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The copper-dependent ACE1 transcription factor activates the transcription of the mco1 gene from the basidiomycete Phanerochaete chrysosporium, by P. Canessa, J. M. Álvarez, R. Polanco, P. Bull and R. Vicuña

Microbiology vol. 154, part 2, pp. 491 - 499

Fig. S1. In vitro transcription-translation of ACE1 transcription factors from S. cerevisiae and P. chrysosporium. (A) (Top) Ethidium-bromide staining of the in vitro synthesized Sc-ace1 mRNA (680 nt) (1) and Pc-ace1 mRNA (1960 nt) (2). (Bottom) Autoradiography of the in vitro-synthesized Sc-ACE1 (1) and Pc-ACE1 (2) transcription factors (B) Binding assays conducted with the 100 bp DNA probe (ACEcup1) shown in Fig. 3 of the main paper and the in vitro synthesized Sc-ACE1. Lanes contain: (1) free probe (FP); (2) standard binding reaction (St); (3-5) binding assays in the presence of 25-, 50- or 100-fold excess, respectively, of unlabelled specific DNA probe; (6-8) binding assays in the presence of 25-, 50- or 100-fold excess, respectively, of a 101 bp unrelated DNA as a non-specific competitor. [PDF] (524 kb)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef