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Supplementary data

The yejABEF operon of Salmonella confers resistance to antimicrobial peptides and contributes to its virulence, by S. M. Eswarappa, K. K. Panguluri, M. Hensel and D. Chakravortty

Microbiology vol. 154, part 2, pp. 666-678

Fig. S1. Susceptibility the of the ΔyejF strain (open circles) to different concentrations of melittin (a) and polymyxin B (b). The assay was done as described in Methods. *P<0.01 (Student’s t test). Filled circles, WT.

Fig. S2. Intracellular proliferation of the ΔyejF in PMA (100 ng ml^-1)-activated U937 cells. The assay was done as described in Methods. **P<0.005 (Student’s t test).

Fig. S3. Co-localization of Salmonella with HD-5 (green) inside Intestine 407 cells at 2 h after infection. The experiment was done as described in Methods. Bar, 2 μm. Red, Salmonella LPS.

Fig. S4. Expression of (a) HD-5 and (b) HBD-1 in Intestine 407 cells. Total RNA was isolated from Intestine 407 cells and reverse-transcribed. The cDNA obtained was used for PCR.

Table S1. Primers used in this study.

Table S2. Amino acid sequence comparison of proteins encoded by the yej operon with components of other peptide transporters of Salmonella.

Table S3. Eukaryotic cell lines used in this study.

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