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Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe, by T. Iwaki, H. Iefuji, Y. Hiraga, A. Hosomi, T. Morita, Y. Giga-Hama and K. Takegawa

Microbiology vol. 154, part 3, pp. 830 - 841

Fig. S1. Comparison of fission yeast (Sp) and budding yeast (Sc) Erg proteins. The black boxes indicate the conserved regions between Sp and Sc Erg proteins. The percentages of identical residues determined by BLAST are also indicated. [PDF] (12 kb)

Fig. S2. HPLC separation of sterols from ARC039 (wild-type, WT), erg31Δ and erg32Δ single mutants. The dashed line indicates the retention time of ergosterol. [PDF] (229 kb)

Fig. S3. Localization of ABC transporters in ergΔ cells. Plasmids pTN197/pmd1 and pTN197/bfr1 were used to express a GFP-tagged ABC transporter (Iwaki et al., 2006). (a) Localization of Pmd1-GFP. Cells carrying pTN197/pmd1 were cultured in minimal medium without leucine and thiamine for 20 h. Pmd1-GFP was found in the plasma membrane of ergΔ cells. (b) Localization of Bfr1-GFP. Cells carrying pTN197/bfr1 were cultured in minimal medium without leucine and thiamine for 20 h. Bfr1-GFP localized to the plasma membrane in ergΔ cells. [PDF] (170 kb)

REFERENCE
Iwaki, T., Giga-Hama, Y. & Takegawa, K. (2006). A survey of all 11 ABC transporters in fission yeast: two novel ABC transporers are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant. Microbiology 152, 2309-2321.

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef