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Acidic stress induces autolysis by a CSP-independent ComE pathway in Streptococcus pneumoniae, by G. E. Piñas, P. R. Cortes, A. G. Albarracín Orio and J. R. Echenique

Microbiology vol. 154, part 5, pp. 1300 - 1308

Table S1. Bacterial strains and plasmids used in this work. [PDF] (73 kb)

Table S2. Primers used in this work. [PDF] (18 kb)

Fig. S1. Kinetics of recA transcripts in response to acidic stress. The recA transcription level was measured in cells exposed to pH 5.9. Strains RC852 (comA lytA), RC820 (lytA) and RC862 (comD^cup3 lytA) were grown in ABM/pH 7.8 to mid-exponential phase and resuspended in ABM/pH 5.9 and RNA was extracted at 0 min, 10 min and 30 min. Fold change in gene expression was measured by quantitative real-time RT-PCR and was calculated using the 2^-ΔΔCT method. The gyrB gene was used as the internal control and the reference condition was time 0 min of strain RC852. Error bars indicate the standard deviation of the mean. [PDF] (58 kb)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef