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Laboratoire de Microbiologie et Génétique Moléculaire, AgroParisTech, INRA, UMR1238, CNRS, UMR2585, F-78850 Thiverval-Grignon, France
Correspondence
Sylvie Blanchin-Roland
Sylvie.Blanchin-Roland{at}grignon.inra.fr
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ABSTRACT |
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 TOP ABSTRACT INTRODUCTIONMETHODSRESULTS AND DISCUSSIONREFERENCES |
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mutant has a less extreme phenotype than other mutants of the pathway, whereas rim9 mutants in Saccharomyces cerevisiae and Candida albicans reportedly exhibit a tight Rim phenotype. Deletion of the long C-terminal tail of YlRim9p/PalI had no phenotypic effect on ambient pH signalling. We also show that the Y. lipolytica PalC orthologue, named YlRim23p, is absolutely required for the alkaline pH response. Its only interactant identified in a genome-wide two-hybrid screen is YlSnf7/Vps32p, confirming the link between the Rim and the Vps pathways. YlRim13p and YlRim20p both interact with YlSnf7/Vps32p but not with YlRim23p. The long C-terminal tail of YlRim9p/PalI interacts neither with YlRim23p nor with YlSnf7/Vps32p. These results show that YlRim23p is a bona fide component of the Rim pathway in Y. lipolytica and that it participates in the complexes linking pH signalling and endocytosis.
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INTRODUCTION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONREFERENCES |
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; Ogrydziak, 1993). In Candida albicans, the genes encoding the three proteases Sap4p, Sap5p and Sap6p are under the control of extracellular pH, Sap5p being required for E-cadherin degradation during mucosal tissue invasion (Villar et al., 2007).
These genes are regulated by a conserved pH signalling pathway called Pal in filamentous fungi and Rim in yeasts. This pathway has been intensively investigated in Aspergillus nidulans (Penalva & Arst, 2002; Herranz et al., 2005; Hervas-Aguilar et al., 2007; Galindo et al., 2007; Penas et al., 2007; Calcagno-Pizarelli et al., 2007), in Y. lipolytica (Lambert et al., 1997; Tréton et al., 2000; Gonzalez-Lopez et al., 2002; Blanchin-Roland et al., 2005), in several other ascomycetes, including Saccharomyces cerevisiae (Li & Mitchell, 1997; Xu & Mitchell, 2001; Vincent et al., 2003; Xu et al., 2004; Boysen & Mitchell, 2006), in C. albicans (Ramon & Fonzi, 2003; Li et al., 2004; Kullas et al., 2004; Cornet et al., 2005; Barwell et al., 2005; Baek et al., 2006), in the basidiomycete Ustilago maydis (Arechiga-Carvajal & Ruiz-Herrera, 2005) and in other fungi (Penalva & Arst, 2002, 2004). At alkaline pH, a cascade of six pal or RIM genes activates the zinc-finger transcriptional factor PacC/Rim101p through a complex C-terminal proteolytic processing event (see below). Previous reports have established that components of the endocytic trafficking cooperate with the Rim pathway in the recruitment of Rim20p and its targeting to Rim101p. Endosomal sorting complex required for transport (ESCRT)-I, -II and -III are three protein complexes that act sequentially in the sorting of both ubiquitinated membrane protein and biosynthetic cargos to the multivesicular body (MVB) vesicles, which are ultimately delivered to the lumen of the vacuole for degradation (Hurley & Emr, 2006). ESCRT-III proteins Snf7/Vps32p and Vps20p and all ESCRT-I and -II components are required for Rim101p activation in S. cerevisiae (Xu et al., 2004; Rothfels et al., 2005; Hayashi et al., 2005). This functional link between the Rim and Vps pathways is conserved in C. albicans (Xu et al., 2004; Kullas et al., 2004; Cornet et al., 2005) and Y. lipolytica (Blanchin-Roland et al., 2005). The PacC/Rim101p truncated form is able to activate alkaline pH-responsive genes and to repress acid-induced genes (for reviews see Penalva & Arst, 2002, 2004). At acidic pH, the Rim/Pal pathway is admittedly inactive, although a specific processing event of Rim101p has been reported in C. albicans (Li et al., 2004). In Y. lipolytica, the Rim pathway seemed to be indirectly required at acidic pH for the activation of the acid-responsive AXP1 gene (Gonzalez-Lopez et al., 2002).
Besides its major role in the ambient alkaline pH response and growth, the Rim pathway appears essential for mating and sporulation in Y. lipolytica (Lambert et al., 1997) and it controls sporulation in S. cerevisiae by activating IME1 expression (Li & Mitchell, 1997) and corepressing, with Nrg1, DIT1 expression in mitotic cells (Rothfels et al., 2005). It also regulates cell differentiation during haploid invasive growth and diploid pseudohyphal differentiation in S. cerevisiae (Lamb & Mitchell, 2003). It is required for the hyphal formation at alkaline pH in C. albicans, in particular by repressing NRG1 expression in a pH-dependent way (El Barkani et al., 2000; Lotz et al., 2004). Moreover, the resistance of cells to high levels of Ca2+ and Li+ requires activity of the Rim pathway, and the subset of ESCRT components needed for Rim101p processing in S. cerevisiae (Lamb & Mitchell, 2003; Platara et al., 2006), in C. albicans (Kullas et al., 2007) and in Fusarium oxysporum (Caracuel et al., 2003). In addition, the Rim pathway participates directly in cell-wall assembly, and acts in parallel with the protein kinase C (PKC) pathway in S. cerevisiae (Castrejon et al., 2006). In C. albicans, this pathway regulates PHR1 and PHR2, encoding two isoforms of the cell-wall glycosidases important for adaptation to ambient pH (Muhlschlegel & Fonzi, 1997), and represses the acid-expressed cell-wall gene RBR1, encoding a glycosylphosphatidylisositol (GPI) protein required for filamentation (Lotz et al., 2004). Finally, defects in ambient pH response lead to reduced virulence in C. albicans, A. nidulans and other pathogenic fungi (Penalva & Arst, 2002; Davis, 2003; Bignell et al., 2005; Cornet et al., 2005; Mitchell et al., 2007; Villar et al., 2007).
According to the current model, largely elaborated through studies in A. nidulans, the pH signalling pathway is organized into two complexes: a plasma-membrane complex, including Rim21p/PalH, Rim9p/PalI and Rim8p/PalF, and an endosomal-membrane complex, including Rim20p/PalA, Snf7/Vps32p, Rim13p/PalB, to which Rim101p/PacC is recruited for its proteolytic activation. The arrestin-like protein PalF (Rim8p) binds strongly to two regions within the C-terminal cytosolic tail of the 7TM PalH protein, which is the likely pH sensor (Herranz et al., 2005). At alkaline pH, PalF is phosphorylated and ubiquitinated in a pH- and PalH-dependent manner. These PalF modifications are partially dependent on the 3TM protein PalI, but independent of the three other Pal proteins: PalA, PalB and PalC (Herranz et al., 2005). Recently, PalI (Rim9p) was shown to localize to the plasma membrane where it may assist localization of PalH (Calcagno-Pizarelli et al., 2007). PalF phosphorylation and ubiquitination may lead to the endocytosis of the PalF–PalH complex, transducing the signal to the downstream complex (Herranz et al., 2005). Several reports in A. nidulans, S. cerevisiae and C. albicans clearly show that PalA/Rim20p interacts with both Snf7/Vps32p, a subunit of ESCRT-III (see above), and PacC/Rim101p (Vincent et al., 2003; Xu & Mitchell, 2001). Both Rim20p–Rim101p and Rim20p–Snf7/Vps32p interactions are required for Rim101p processing. An interaction between the calpain-like protease Rim13p/PalB and Snf7/Vps32p was detected during one whole-genome screen of two-hybrid interactants in S. cerevisiae (Ito et al., 2001), but no direct interaction between Rim13p/PalB and Rim101p/PacC has been reported. In A. nidulans, PalB was recently shown to be the ‘signalling’ protease that catalyses the first, pH-regulated cleavage of PacC (Penas et al., 2007), whereas the second processing step involves the proteasome (Hervas-Aguilar et al., 2007).
PalC was the only Pal protein with no homologue in hemiascomycetous yeasts, except Y. lipolytica. However, recently, YGR122w was identified as a likely S. cerevisiae orthologue (Galindo et al., 2007). Ygr122wp was previously shown to bind Snf7/Vps32p (Ito et al., 2001; Uetz et al., 2000) and to be required for activation of Rim101p (Rothfels et al., 2005; Barwell et al., 2005), and ygr122w results in Li+ hypersensitivity (Galindo et al., 2007). A mutational analysis of PalC revealed conserved regions, one potentially functional as a BRO1 domain (Tilburn et al., 2005). While this manuscript was in preparation, PalC was shown to localize to cortical structures at alkaline pH and to bind Snf7/Vps32p (Galindo et al., 2007).
In this paper, we show that YlRIM23, the orthologue of PalC, is required for the pH response as well as for mating in Y. lipolytica. We also report that YlRim23p binds to YlSnf7/Vps32p and confirm that YlSnf7/Vps32p binds to both YlRim13p and YlRim20p. We further show that the long C-terminal tail of YlRim9p does not seem to be involved in the pH response.
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METHODS |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONREFERENCES |
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. Unless otherwise stated, sequence data were obtained from the complete genome assembly of Y. lipolytica (http://cbi.labri.fr/Genolevures; Dujon et al., 2004).
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). Solid and liquid media were buffered at pH 4.0 (with 0.2 M citrate buffer), at pH 7.0 (with 0.2 M sodium phosphate buffer) or at pH 8.0 (with 25 mM Tris/HCl) and supplemented with 0.1 g uracil l–1. The pH of liquid media was stable within 0.3 pH units during growth. Growth at various pHs was assayed as previously described (Blanchin-Roland et al., 2005). Estimation of efficiency of mating for compatible Y. lipolytica auxotrophs was done as described by Lambert et al. (1997).
DNA and RNA techniques.
Standard recombinant DNA techniques were performed essentially as previously described (Gonzalez-Lopez et al., 2002). PCR products were routinely checked by sequencing; sequences were obtained from the DNA sequencing department of Genome Express. They were assembled and annotated using the GCG package (University of Wisconsin, Madison, WI, USA). All transformation events were checked by colony PCR and confirmed by Southern analysis. Gene expression was determined by real-time quantitative reverse transcriptase-PCR as previously described (Blanchin-Roland et al., 2005).
Deletion of YlRIM23 and YlRIM9.
The rim23 : : URA3 and the rim9 : : URA3 deletion cassettes were constructed according to Fickers et al. (2003). The promoter and terminator regions of each gene were amplified using primers RIM23FP1/RIM23P2+RIM23T1/RIM23HT2 or RIM9CP1/RIM9P2+RIM9T1/RIM9ET2, respectively (see Table 2 for primer sequences). For each gene, the resulting fragments were annealed and amplified with the flanking primers. These cassettes were cloned into pBSKS+ to generate plasmids BSPTRIM23 and BSPTRIM9, respectively. The loxR-URA3-loxP module (Fickers et al., 2003) was inserted between promoter and terminator sequences at the I-SceI site, generating plasmids pINA1375 and pINA1359, respectively. The cassettes were excised from these plasmids and purified with the QIAquick gel extraction kit (Qiagen). The RIM9 deletion cassette was integrated by double crossover at the YlRIM9 locus of the SY12 strain to give four independent clones of the SY1375 strain (Ylrim9-13 allele). The RIM23 deletion cassette was similarly used to generate two independent clones of the SY1359 strain (Ylrim23-10 allele). YlURA3 was excised from the SY1375 strain by using the plasmid pUB4-CRE (Fickers et al., 2003) to give the SY1375u strain.
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-13 background and in the SY1372u strain carrying the YlRIM9SL allele (see below), a deletion cassette was constructed from the loxR-Hph-loxP module (Fickers et al., 2003) and cloned into BSPTRIM23 at the I-SceI site, to generate plasmid pINA1387. The deletion cassette was excised from pINA1387, purified as above, then integrated by double crossover at the YlRIM23 locus into the SY1372u strain to generate five independent clones of the SY1389 strain (YlRIM9SL, Ylrim23-31) and into the SY1375u strain to generate four independent clones of the SY1390 strain (Ylrim9-13, Ylrim23-31).
Subcloning of the YlRIM9 gene.
The complete YlRIM9 ORF flanked by 660 bp of upstream and 544 bp of downstream sequences was rescued from plasmid AW0AA029A08 (AL414126). To increase the size of the upstream sequence, a PCR product was amplified from SY12 genomic DNA using primers RIM91 and RIM92r (Table 2
). Both this PCR product and the fragment carrying RIM9 were inserted into the integrative URA3 plasmid pINA300' to give pINA1371.
Construction of the mutant encoding a C-terminally truncated YlRim9p.
The YlRIM9SL-1372 allele was obtained by overlapping PCR and contains two mutations, A889T and C894G, which replace the Arg297 and Tyr298 codons by nonsense codons within the RIM9 coding sequence. PCR products were obtained from genomic DNA with primer pairs RIM9CP1-RIM9end3r and RIM9end5-RIM9ET2 (Table 2), mixed and amplified with flanking primers to generate a full-length product containing the internal mutations. This PCR product was inserted into pINA1371 to generate plasmid pINA1372, which was checked by sequencing. The BamHI-digested pINA1372 was targeted to the promoter of RIM9 in the SY1375u strain (Ylrim9-13 allele) to give three independent clones of the SY1372 strain (YlRIM9SL allele). BamHI-digested pINA1371 was similarly targeted in the SY1375u strain to give three independent clones of the SY1371 strain (YlRIM9 allele). YlURA3 was excised from the SY1372 strain, using pUB4-CRE as above, to give the SY1372u strain.
Yeast two-hybrid assay.
S. cerevisiae PJ69-4A (Table 1) and plasmids pAS2 (TRP1, bla) and pACT2 (LEU2, bla) were used for the two-hybrid analysis (James et al., 1996). Primers were designed to amplify the complete RIM8 nucleotide sequence (RIM8DHSf and RIM8DHBa), the complete RIM13 nucleotide sequence (RIM13DHNc and RIM13DHBa), the complete RIM23 nucleotide sequence (RIM23DHNc and RIM23DHBa) and the C-terminal RIM9 coding sequence from codon 254 to the stop codon 724 (RIM9CDHNc and RIM9CDHBa) (Table 2). The PCR products were digested by NcoI (or SfiI in the case of RIM8) and BamHI to allow in-frame cloning in the multisite of each two-hybrid vector. The recombinant vectors carrying the complete RIM20 nucleotide sequence were constructed by B. Tréton (unpublished). Empty and recombinant two-hybrid vectors were co-transformed into yeast cells and transformants were selected on minimal medium plus histidine, methionine, uracil and adenine for selection of the plasmids. Purified transformants were streaked on YNB plus histidine, methionine and uracil, on YNB plus adenine, methionine, uracil and 5 mM 3-aminotriazole or on YNB plus methionine and uracil for direct selection of the interactants (James et al., 1996).
Two-hybrid library screening.
The PJ69-4
strain was transformed with pAS2-RIM23 (see above), and a mid-exponential culture (120 ml) of the resulting transformant was mixed with an aliquot of each of the three Y. lipolytica two-hybrid libraries constructed in pGAD-C1 to C3 plasmids in PJ69-4A (Kabani et al., 2000) at a ratio of 3 : 1 (bait : prey). After mating at 30 °C for 16 h, cells were harvested and plated onto YNB plus adenine, methionine, uracil and 5 mM 3-aminotriazole for selection of His+ colonies. The number of diploid and parental cells was determined as previously described (Kabani et al., 2000). The number of diploids obtained (BC1, 9.3x107; BC2, 4.7x109; BC3, 7.5x105) was sufficient to ensure a representative sampling of the two first pools of the library (107 clones per pool). After 7–8 days at 30 °C, His+ diploids were replica-plated onto YNB plus histidine, methionine and uracil plates. Ade+ colonies were recovered from the third to the eighth day and streaked twice on YNB plus methionine and uracil plates to retain Ade+ His+ diploids.
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RESULTS AND DISCUSSION |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONREFERENCES |
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; Gonzalez-Lopez et al., 2002). A putative orthologue (YALI0F17028 g) of palC was identified from the Y. lipolytica genome (Dujon et al., 2004; Tilburn et al., 2005). The predicted 441 aa protein was named YlRim23p. A likely PalC orthologue, encoded by YGR122w, was recently identified in S. cerevisiae (Galindo et al., 2007). Moreover, PalH has two homologues in S. cerevisiae and C. albicans, Rim21p and Dfg16p (Barwell et al., 2005), but only one in Y. lipolytica, Rim21p. We found two putative paralogues of YlRim21p (YALI0B22814g and YALI0B06710g) in the Y. lipolytica genome. Deletion of each of these ORFs had no effect on pH response (not shown). This suggests that the Y. lipolytica genome carries only one functional equivalent of PalH/YlRim21 and none of ScDfg16p.
To check if YlRIM23 was involved in ambient pH signalling, the SY1359 strain carrying the null mutation (Ylrim23-10) was created (see Methods). We previously observed that the Ylrim9-D7 insertional mutant had a milder effect than other rim mutants on growth or PHR1 expression at alkaline pH (Gonzalez-Lopez et al., 2002). To check whether the same leaky phenotype was displayed by a null mutant, the SY1375 strain carrying the Ylrim9-13 allele was constructed (see Methods). The effects of YlRIM23 or YlRIM9 deletion on the transcription of different pH-regulated genes were assessed by real-time quantitative PCR on total RNAs extracted from cultures grown in Y medium at pH 4.0 and pH 8.0, using actin transcript as a reference. As ambient alkaline pH-responding genes, we chose XPR2 and YlPHR1 and as acidic pH-responding gene, YlPHR2 (Blanchin-Roland et al., 2005).
At pH 8.0, both the Ylrim23-10 and Ylrim9-13 mutants nearly abolished XPR2 transcription, and the Ylrim23-10 mutant reduced YlPHR1 expression about 15-fold, whereas the Ylrim9-13 mutant reduced it about 6-fold (Fig. 1). These observations were confirmed by measuring the expression of the XPR2' : lacZ fusion integrated at the XPR2 locus of these strains: the residual β-galactosidase activity was about 1.4 % for Ylrim23-10 and 14.6 % for Ylrim9-13. At pH 4.0, no or very low transcription of these alkaline reporter genes could be detected (Fig. 1). At alkaline pH, in the Ylrim23-10 mutant as in the Ylrim9-13 mutant, expression of the acid-induced gene YlPHR2 was derepressed more than 38-fold compared to the wild-type level at the same pH, reaching its level at acidic pH in the reference strain in the case of Ylrim9-13 or exceeding it in the case of Ylrim23-10 (Fig. 1). Taken together, these results strongly suggest that, like other YlRIM genes (Gonzalez-Lopez et al., 2002; Lambert et al., 1997), YlRIM23 is absolutely required for transcriptional activation of alkaline-induced genes and for repression of acid-induced genes at pH 8.0. The Ylrim9-13 mutant, by contrast, led to a somewhat less extreme phenotype, as previously stated (Gonzalez-Lopez et al., 2002).
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-10 induced the characteristic growth defect of Rim mutants, affecting growth at alkaline pH but not at neutral or acidic pH (Fig. 2) (Gonzalez-Lopez et al., 2002). As previously observed for the insertional rim9-D7 mutant (Gonzalez-Lopez et al., 2002), the Ylrim9-13 mutation had a less pronounced effect on growth at alkaline pH.
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leads to a less extreme phenotype than other Ylrim mutations, as measured by activation of alkaline-induced genes, repression of an acid-induced gene (Fig. 1), growth at alkaline pH (Fig. 2) and efficiency of mating (see below), confirming the data obtained with the insertional mutant described by Gonzalez-Lopez et al. (2002). In a similar way, a palI null mutation in A. nidulans allows partial growth at pH 8.0, whereas all other pal mutations completely prevent growth at this pH (Arst et al., 1994; Denison et al., 1998). The phenotype of rim9 mutants in yeasts has been poorly investigated up to now. In S. cerevisiae, rim9 mutants exhibit a tight defect in sporulation (Su & Mitchell, 1993); in C. albicans, the rim9 –/– mutant displays a tight defect in ambient pH response (M. Cornet, personal communication).
The long YlRim9p C terminus does not seem to be involved in the pH response
Alignment of PalI/Rim9p sequences showed that YlRim9p (742 aa) (Gonzalez-Lopez et al., 2006) and its orthologues in filamentous fungi are much longer than their orthologues in other yeasts (239 aa for ScRim9p). Strong sequence conservation is restricted to the regions including the hydrophobic, putative transmembrane segments. The highly hydrophilic C-terminal tails that lie outside the region of homology between all the PalI/Rim9p sequences represent about 75 % of YlRim9p, 66 % of PalI, 82 % of MgPalI and 72 % of NcPalI. We noticed that deletion of a long Rim9p/PalI in Y. lipolytica and filamentous fungi results in a leaky phenotype, whereas the short Rim9p appears absolutely required in S. cerevisiae and C. albicans. Although we failed to detect any structural or functional patterns within these poorly conserved sequences, we wondered whether this large C-terminal tail in YlRim9p played a role in the pH response. To address this question, we established the phenotype of a mutant expressing a C-terminally truncated YlRim9 polypeptide. Point mutations were introduced to replace the adjacent residues R297 and Y298 by nonsense codons (YlRIM9SL allele, see Methods). This construct replaced the wild-type gene at its normal location (SY1372 strain). As a control, the wild-type allele was used to replace the null mutation Ylrim9-13 (SY1371 strain). The effects of the C-terminally truncated form of YlRim9p on the transcription of different pH-regulated genes were assessed by real-time quantitative PCR at pH 4.0 and pH 8.0. At pH 8.0, identical levels of XPR2 or YlPHR1 transcripts were measured in SY1372 (YlRIM9SL mutation) and in the isogenic control SY1371. They were reduced by 30 % and 6 % for XPR2 and YlPHR1, respectively, compared to the wild-type strain, reflecting unidentified background differences (Fig. 1). These observations were fully confirmed by measuring the expression of the XPR2' : lacZ fusion integrated at the XPR2 locus of SY1372 and SY1371 (72 % of residual β-galactosidase activity for both strains). At alkaline pH, in SY1372 as in SY1371, expression of the acid-induced gene YlPHR2 was also comparable to wild-type levels at the same pH (Fig. 1). At pH 4.0, the YlRIM9SL mutation had no effect on the transcription of these alkaline- or acid-responsive genes (Fig. 1). Taken together, these results strongly suggest that the C-terminal tail of YlRim9p is not required for transcriptional activation of alkaline-induced genes and for repression of acid-induced genes at pH 8.0.
Both Y. lipolytica and filamentous fungi carry a conserved YlRIM23/PalC gene, besides a long version of YlRIM9/PalI. We wondered whether YlRim23p/PalC may counteract a putative negative effect of the YlRim9p/PalI C-terminal tail on pH signal transduction. According to this hypothesis, the null rim23 mutant in the RIM9SL context should behave like the wild-type strain. RIM23 was also deleted in the rim9-13 mutant. All clones of SY1389 (rim23-1, RIM9SL) and of SY1390 (rim23-31, rim9-13) exhibited low levels of XPR2-driven β-galactosidase activity at pH 8.0 (about 0.03 % of wild-type levels). Transcription of alkaline- or acidic-responsive genes was affected in a similar way and to the same extent by the double mutations or by the single mutation Ylrim23-10 (see Fig. 1). The double mutations induced the characteristic growth defect of Rim mutants, drastically crippling growth at alkaline but not at neutral pH (Fig. 2). Taken together, these results show that YlRIM23 is absolutely required for the RIM101-dependent pH response, but does not counteract a putative negative effect of the YlRim9p C-terminal tail on pH signal transduction. This indicates that in Y. lipolytica at least, and possibly in filamentous fungi, the long C-terminal tail of YlRim9p/PalI does not play a major role in the pH response and that its presence is not related to that of YlRim23p/PalC.
Mating in Y. lipolytica requires YlRim23p and, to a lesser extent, YlRim9p
We previously reported that mating in Y. lipolytica requires activation of the Rim pathway in both parents. Mutants affecting RIM8, RIM13, RIM20, or RIM21 mated less efficiently than the control strains, yielding few pal/+ and even fewer pal/pal diploids (Lambert et al., 1997). As shown in Table 3, efficiency of mating was drastically reduced compared to wild-type contexts in +xrim23-10 crosses, reduced in +xrim8-21 and slightly reduced in +xrim9-13. The RIM9SL C-terminal truncation alone did not affect efficiency of mating whereas no diploids could be obtained from +xrim23-1, RIM9SL and +xrim23-31, rim9-13 confrontations. This indicates that mating requires Rim23 like other Rim factors indicated above, and to a lesser extent Rim9, but not the C-terminal tail of Rim9p.
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). Exhaustive matings against three genomic libraries (Kabani et al., 2000), yielded 19, 175 and 68 His+ Ade+ diploids, respectively (see Methods). The inserted genomic fragments were directly amplified from yeast colonies by PCR and sequenced. From 132 sequences out of a total of 149 analysed, redundant and overlapping fragments were found for only one ORF, which is a strong argument for a true interaction. This ORF matches the YlSNF7/VPS32 gene (accession number YALI0C16027g), hereafter designated SNF7 for simplicity. The fusion points were generated by either TaqI or HpaII, which are the two ‘CG’ enzymes cutting in the promoter sequence in-frame with the coding sequence, at positions –75 bp and – 69 bp relative to the A of ATG, respectively. No fusion point within the coding sequence was generated by the ‘CG’ enzymes. This suggests that at least the N-terminal region of Snf7 is required for the interaction with Rim23p. No other candidate protein was repeatedly obtained in this screening. Plasmids were purified from one representative clone for each fusion point and co-transformed together with either pAS2 or pAS2-RIM23 in the PJ69-4A strain. After checking the expression of the HIS3 and ADE2 reporter genes, the clones turned out to be true positives while all the controls were negative (Fig. 3a, A and B). These results indicated no auto-activation of the expression of the reporter genes and reproduced the interaction of Snf7p with Rim23p. This result is in line with recently published data from A. nidulans demonstrating by two-hybrid and recombinant protein pull-down assays that PalC binds Snf7p (Galindo et al., 2007). PalC was recently shown to be recruited to plasma-membrane-associated structures in an alkaline pH- and PalH-dependent but PalA-independent manner, which led to the suggestion that PalC can link the putative pH-sensing complex and the endosomal complex (Galindo et al., 2007).
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, C and D). Fig. 3(b) summarizes the network of two-hybrid interactions tested. These assays confirm the interactions between Snf7p and Rim20p/PalA/AIP1/Alix already described in S. cerevisiae, A. nidulans and C. albicans (Ito et al., 2001; Vincent et al., 2003) and between Snf7p and Rim13p, revealed only by one whole-genome screen of two-hybrid interactants in S. cerevisiae (Ito et al., 2001). They also suggest the absence of interaction between YlRim23p and any of the other Rim factors tested.
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ACKNOWLEDGEMENTS |
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Edited by: M. Molina
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REFERENCES |
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TOPABSTRACTINTRODUCTIONMETHODSRESULTS AND DISCUSSIONREFERENCES |
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Received 22 January 2008;
revised 29 February 2008;
accepted 5 March 2008.
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