Polar accumulation of the metabolic sensory histidine kinases DcuS and CitA in Escherichia coli, by P. Scheu, S. Sdorra, Y.-F. Liao, M. Wegner, T. Basché, G. Unden and W. Erker
Microbiology vol. 154, part 8, pp. 2463 - 2472
The supplementary data file [PDF] (354 kb) contains experimental results confirming the formation of fluorescent DcuS-YFP fusion protein by fluorescence spectroscopy, showing the intensity ratios versus z-position of the focus, and demonstrating that polar accumulation responds to the presence of effector. It includes the following figures:
Fig. S1. Fluorescence emission spectra of DcuS-YFP fusion protein.
Fig. S2. Dependence of the intensity ratio on the focus z-position.
Fig. S3. Influence of effectors fumarate and citrate on the polar accumulation of DcuS-YFP and CitA-YFP.
Four QuickTime movie files are also available:
Movie 1: 3D-reconstruction of an E. coli cell expressing DcuS-YFP
(strain JM109/pMW407; in the presence of 20 mM fumarate; custom-built microscope) [QuickTime file] (698 kb)
Movies 2a and 2b: 3D-reconstruction of an E. coli cell expressing DcuS-YFP
(strain UU1250/pMW407; without fumarate; custom-built microscope). Movies 2a [QuickTime file] (1571 kb)
and 2b [QuickTime file] (1300 kb)
show the same cells but differ with respect to the thresholds.
Movie 3: 3D-reconstruction of an E. coli cell expressing DcuS-YFP
(strain JM109pMW407; without fumarate; commercial microscope) [QuickTime file] (1393 kb)
Movie 4: 3D-reconstruction of an E. coli cell expressing DcuS-YFP
(strain JM109/pMW407; without fumarate; commercial microscope) [QuickTime file] (2000 kb)
Copyright © 2009 Society for General Microbiology.
