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Pathway for H₂O₂ and O₂ detoxification in Clostridium acetobutylicum, by O. Riebe, R.-J. Fischer, D. A. Wampler, D. M. Kurtz Jr and H. Bahl

Microbiology vol. 155, part 1, pp. 16 - 24

Fig. S1. Reductive titration of revRbr by NADPH/Rd and H₂O₂ reoxidation. The top panel shows the UV-vis absorption spectrum of as-isolated revRbr (100 μM, highest absorbance at 490 nm) containing FNR and Rd (0.5 μM each) in anaerobic 50 mM MOPS pH 7.3 and the spectral changes upon successive additions of substoichiometric NADPH until no further absorbance changes occurred (lowest absorbance at 490 nm). The bottom panel shows the spectrum of the same solution as in the top panel after the NADPH titration (lowest absorption at 490 nm), and the subsequent spectral changes upon additions of substoichiometric H₂O₂ until no further changes occurred (highest absorbance at 490 nm). Arrows show directions of absorbance changes during the titrations. [PDF] (408 kb)

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