Supplementary figures
Identification of genes required for different stages of dendritic swarming in Bacillus subtilis, with a novel role for phrC, by K. Hamze, D. Julkowska, S. Autret, K. Hinc, K. Nagorska, A. Sekowska, I. B. Holland and S. J. Séror
Microbiology vol. 155, part 2, pp. 398-412
Fig. S1. Scheme of regulatory circuits controlling surfactin production and competence expression in liquid cultures. ComPA constitutes a two-component signal transduction system controlled by the interaction of the secreted peptide pheromone, ComX, with the histidine kinase ComP. In addition, the secreted pentapeptide CSF, ultimately re-enters the cell, via the Opp permease, in order to modulate the inhibition of the response regulator, ComAp by the RapC phosphatase. ComA, also possibly phosphorylated by acetyl phosphate, regulates a large number of genes in addition to srfA. The srfA promoter is subject to multiple regulation and, in addition to the expression of the surfactin synthases (a, b, c) Directs the expression of comS (competence activator) embedded in an alternative reading frame in srfB. Following production, surfactin is secreted to the medium probably involving an ABC transporter. ComS negatively regulates the ability of MecA to target ComK (competence regulon transcription factor) for proteolysis. Other relevant players indicated in these networks include the global regulators AbrB, CodY (GTP sensor) and DegU (Mäder et al., 2002), the oxidative stress transcription factor Spx (Nakano et al., 2002), Hag (flagellin) and ComQ (modification and export of ComX). Black boxes represent genes. Black arrows and black bars represent positive and negative regulators, respectively.
Fig. S2. Surfactin-independent swarming patterns on LB: mutants comA and phrC are defective in swarming. (a) 168 swrA sfp; (b) 168 swrA sfp comA; (c) 168 swrA sfp phrC. All swarms were incubated 16 h ; swarming is essentially blocked early in both mutants. Bar, 1 cm.
References
Mäder, U., Antelmann, H., Buder, T., Dahl, M. K., Hecker, M. & Homuth, G. (2002). Bacillus subtilis functional genomics: genome-wide analysis of the DegS-DegU regulon by transcriptomics and proteomics. Mol Genet Genomics 268, 455-467.
Nakano, S., Zheng, G., Nakano, M. M. & Zuber, P. (2002). Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis. J Bacteriol 184, 3664-3670.
Copyright © 2009 Society for General Microbiology.
