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Molecular identification, typing and traceability of cyanobacteria from freshwater reservoirs, by E. Valério, L. Chambel, S. Paulino, N. Faria, P. Pereira and R. Tenreiro

Microbiology vol. 155, part 2, pp. 642 - 656

Table S1. Cyanobacterial isolates used in this study and GenBank accession numbers for 16S rDNA and rpoC1 sequences [PDF] (26 kb)

Table S2. Morphological characteristics of the cyanobacterial species, used to perform their identification [PDF] (480 kb)

Fig. S1. MrBayes tree of the species belonging to the Chroococcales order using 1203 nt of the 16S rRNA gene sequences in the alignment. The posterior probabilities values, above 50, are indicated to provide branch support. Bar: 10% sequence divergence. GenBank accession numbers are indicated after species designation (names in bold-face correspond to sequences determined in this study). [PDF] (330 kb)

Fig. S2. MrBayes tree of the species belonging to the Oscillatoriales order using 1182 nt of the 16S rRNA gene sequences in the alignment. The posterior probabilities values, above 50, are indicated to provide branch support. Bar: 10% sequence divergence. GenBank accession numbers are indicated after species designation (names in bold-face correspond to sequences determined in this study). [PDF] (340 kb)

Fig. S3. MrBayes tree of the species belonging to the Nostocales order using 1203 nt of the 16S rRNA gene sequences in the alignment. The posterior probabilities values, above 50, are indicated to provide branch support. Bar: 10% sequence divergence. GenBank accession numbers are indicated after species designation (names in bold-face correspond to sequences determined in this study). [PDF] (360 kb)

Fig. S4. (a) Global neighbour-joining tree of representative species using 1182 nt of the rRNA gene sequences in the alignment. (b) Neighbour-joining tree of the rpoC1 sequences using 452 nt in the alignment. Percentage bootstrap values of 1000 replicates are given at each node. Bar: 10% sequence divergence. GenBank accession numbers are indicated after species designation (names in bold-face correspond to sequences determined in this study). [PDF] (445 kb)

Fig. S5. (a) Global maximum-parsimony tree of representative species using 1182 nt of the rRNA gene sequences in the alignment. (b) Maximum-parsimony tree of the rpoC1 sequences using 452 nt in the alignment. GenBank accession numbers are indicated after species designation (names in bold-face correspond to sequences determined in this study). [PDF] (415 kb)

Fig. S6. Representative M13 (a) and ERIC (b) fingerprinting profiles of the cyanobacterial species comprising more than five isolates; M: 1 kb plus molecular ladder. [PDF] (1480 kb)

Fig. S7. Composite analysis of hierarchical clustering based on the M13 and ERIC PCR fingerprints of all Microcystis aeruginosa isolates. [PDF] (1220 kb)

This Article Abstract Full Text Services Email this article to a friend Alert me to new issues of the journal Citing Articles Citing Articles via CrossRef